An Integrated Workflow for Cryo-Electron Tomography

Blanca Carbajal González, M.S
Thermo Fisher Scientific
Wednesday April 3, 2019 – 12PM

The ultimate goal of structural and cell biologists is to derive the structures of molecular machines in their natural, functional context.  Cryo-electron tomography is a label-free, fixation-free imaging technique, which provides 3D nanometer-resolution.  However, high resolution cryo-ET has mainly been limited to isolated organelles or the peripheries of cells. With recent advances in thin lamella preparation with the Thermo ScientificTM Aquilos TM Cryo-FIB, researchers are gaining novel insights into previously inaccessible regions of the cell.Here we describe the suite of instruments that enable Thermo Fisher Scientific’s cryo-electron tomography workflow as well as application examples from the recent literature.

2019-04-09T19:26:48+00:00March 29th, 2019|Forums, Past Events|