Krios Usage2019-09-18T18:34:58+00:00

Krios Operations

Instrument Status
Krios01: Normal Operations
Krios02: Normal Operations
Krios03: Normal Operations

General Krios information

1) Technicians will only be available for grid drop off and retrieval from 2-4pm during business days. Note, if you are dropping off unclipped grids, they are due at the center no later than Wednesday @ 2pm the week before the scheduled session. If you are dropping off pre-clipped grids, they are due at the center no later than Friday @ 4pm the week before your scheduled session. We encourage you to drop off grids well before the cut-off time and we accept grids up to 5 weeks ahead of your session.To open the Krios Grid Drop-off Checklist,  click here.

  • If you have already dropped off grids and would like to swap/add grids for a Krios session, they must be swapped/added by Wednesday @ 2pm (if unclipped) or Friday @4pm (if clipped) the week before the scheduled session. These new grids must be accompanied by a new Krios Grid Drop-off Checklist.

If you have unclipped grids for an upcoming Krios session, then you are required to purchase one of two kits:

1. KRIOS CARTRIDGE KIT ($85.00):

The Krios Cartridge Kit contains the supplies sufficient for 1 clipping from SEMC. SEMC staff have assigned grid clipping days and supplies must be presented or paid for at that time for grids to be clipped and scheduled for upcoming Krios time. We DO accept pre-clipped grids in new AutoGrids, and also grids in new AutoGrids that have been previously imaged using a different Krios. However, we reserve the right to inspect these grids and reject them for loading based on their integrity. Used AutoGrids are NOT permitted because they invalidate our warranty and have the potential to result in many weeks of downtime for the instrument.

The Krios Cartridge Kit contains:

  • 4x C-Clips
  • 4x AutoGrids
  • 1x AutoGrid Box

2. KRIOS CARTRIDGE MINI-KIT ($69.00)

The Krios Cartridge Mini-Kit contains all the supplies sufficient for 1 clipping from SEMC EXCEPT the AutoGrid Box. SEMC staff have assigned grid clipping days and supplies must be presented or paid for at that time for grids to be clipped and scheduled for upcoming Krios time. We DO accept pre-clipped grids in new AutoGrids, and also grids in new AutoGrids that have been previously imaged using a different Krios. However, we reserve the right to inspect these grids and reject them for loading based on their integrity. Used AutoGrids are NOT permitted because they invalidate our warranty and have the potential to result in many weeks of downtime for the instrument.

The Krios Cartridge Mini-Kit contains:

  • 4x C-Clips
  • 4x AutoGrids

Payment can be made in one of two ways:

  1. Write a check payable to New York Structural Biology Center, Inc., give it to Ashleigh Raczkowski or Daija Bobe. Ashleigh and Daija can be found in the SEMC lab M-F, 10:00am – 4:00pm. PLEASE PURCHASE YOUR SUPPLIES PRIOR TO YOUR LAB SESSION.
  2. Purchase your kit with a credit card using PayPal.
    GO TO WWW.PAYPAL.COM and click on “Send Money”. Follow the instructions and use your credit card to send money to: Paypalkits@nysbc.org. Please print your receipt and bring it with you to the lab as proof you have paid. PLEASE INCLUDE IN YOUR PAYPAL E-MAIL THE SPECIFIC KIT YOU ARE PURCHASING (i.e., “User Kit”, “Krios Cartridge Kit” or “Krios Cartridge Mini-Kit”). Give your printed receipt to the lab and give a copy to Daija Bobe or Ashleigh Raczkowski. Daija and Ashleigh are available in the SEMC lab M-F, 10:00am – 4:00pm.

2) Each institution has pre-allocated days on instrument under normal operations, but are not limited to this time. There is rapid access time or NYSBC maintenance time each cycle that every user may apply for. 

3) We have two (2) types of rapid access, both of which require an application:

     1. Scheduled rapid access:

In between cycles we have system time for maintenance and development. Unused time during this period may be re-allocated as pre-scheduled rapid access time.

     2. Moment’s notice:

Time available due to an unexpected opening in the schedule. Grids should be pre-clipped and stored at the center, or the user should be able to bring it to the center at a moment’s notice for immediate cassette loading.

For further details on rapid access see an example application click here.

4) The preallocated days are meant to be similar to an airline/train schedule. If time is lost or unassigned then the session is gone. However, there is at least 1 possible rapid access session each cycle to act as an emergency buffer.

5) These dates are the allocation to the institution not an individual lab. Please contact your institution representative for more information.