To start 2018-01-26T21:22:03+00:00

Getting Started

These are the NYSBC-affiliated institutions and institutional representatives:

Institution Representative Email
Albert Einstein College of Medicine Hernando Sosa hsosa@aecom.yu.edu
City University of New York Reza Khayat rkhayat@ccny.cuny.edu
Columbia University Bob Grassucci rg2502@columbia.edu
Memorial Sloan Kettering Cancer Center Chris Lima limac@mskcc.org
Mount Sinai School of Medicine Iban Ubarretxena Iban.Ubarretxena@mssm.edu
New York University David Stokes stokes@nyu.edu
Rockefeller University Seth Darst darst@rockefeller.edu
Wadsworth Center Michael Marko mike.marko.em@gmail.com
Weill Cornell Medical College David Eliezer dae2005@med.cornell.edu

If you are a member of any of these affiliated institutions, these MEMBER INSTITUTION SERVICES are covered by Annual Dues:

  • Advanced instrumentation and expertise for Electron Microscopy
  • Training in instrumentation and techniques, including accredited classes and individual instruction
  • Assigned, dedicated instrument time is part of consortium membership – there are no special fees beyond annual membership dues.

PLEASE NOTE: Annual dues do not cover the laboratory supplies and consumables you will need. See LABORATORY SUPPLIES (below).

If you belong to an NYSBC-affiliated institution

You have access to both the instrumentation and staff resources of the cryo-EM facility at the NYSBC. If you are interested in embarking on an EM project at the Simons Electron Microscopy Center, please follow these steps:

1. CREATE AN ACCOUNT 
Create a SEMC account for yourself with the following form: SEMC Account Registration for yourself. Note: You must create an account from a member institution IP address. Once created you may then log in from anywhere.

2. REGISTER YOUR PROJECT 
Register your project by using your SEMC credentials using the following form: EM Proposal Submission

3. REVIEW OUR INFORMATION 
Read the resources and capabilities of the Simons Electron Microscopy Center on this site: Considerations before beginning a project and Overview of EM Techniques.

4. SCHEDULE A MEETING 
Arrange a meeting with the SEMC staff (EMG@NYSBC.org) and notify your institution’s Operations Committee Member

Laboratory Supplies

You will need a variety of supplies and consumables. SEMC users are responsible for covering the cost of expendable supplies that they use. While there are supplies in the lab and we will do our best to be cooperative so that your project can continue to move forward, you SHOULD NOT ASSUME THAT THE SUPPLIES YOU NEED WILL BE AVAILABLE IF YOU RUN OUT OR DIDN’T BRING THEM WITH YOU. Sometimes the lab might not have what you need on that day.

If you do not have the supplies you need in your home lab, you can purchase them over the internet.  Follow the links in these lists:

NOTE: The Standard User Kit contains the minimal supplies necessary for a project. Your project may have more specific requirements, and you may need to order additional supplies.

These supplies are included in the STANDARD USER KIT:

4.5″ Scissors

http://www.tedpella.com/dissect_html/scissor2.html#13215
http://www.emsdiasum.com/microscopy/products/tweezers/forceps.aspx#72938

Grid Box

http://www.tedpella.com/grids_html/gridbox.htm#anchor1280885
http://www.emsdiasum.com/microscopy/products/grids/accessories.aspx#71150

Dumont Anti-Cap Bio Tweezer

http://www.tedpella.com/twzr-mis_html/twzr-ac.htm
http://www.emsdiasum.com/microscopy/products/tweezers/dumont_negative_action.aspx#02-N5

Dumont Stainless Steel Tweezer

http://www.tedpella.com/twzr-mis_html/twzr-ac.htm
THESE TWEEZERS ARE CURRENTLY AVAILABLE FROM EMS ONLY IN A KIT OF SEVERAL DIFFERENT TWEEZERS

Cryo Grid Box with Lid

http://www.tedpella.com/grids_html/gridbox.htm#_160_405
http://www.emsdiasum.com/microscopy/products/cryofixation/cryo_dewars_flasks.aspx#71166

Dissecting Forceps

http://www.tedpella.com/dissect_html/forceps.htm#5002-29
http://www.emsdiasum.com/microscopy/products/histology/forceps.aspx#77937-21

4 Quantifoil, 200#, 2um, Copper

You will need a constant supply of these. The number “4” is used here because you get 4 if you buy a User Kit.

http://www.emsdiasum.com/microscopy/products/grids/quantifoil.aspx

4 C-Flat Grids, 2um/2um-2C, Copper

You will need a constant supply of these. The number “4” is used here because you get 4 if you buy a User Kit.

http://www.emsdiasum.com/microscopy/products/grids/cflat.aspx#CF

The User Kit comes in a metal lunch box, which you can purchase for yourself here:

http://www.ideastage.com/Medium-Retro-Lunch-Box—Blank-523657438?gclid=Cj0KEQjwz-i3BRDtn53Z5Z7t4PUBEiQA23q2AHlEiCXqXGUNh9k81ecWh640usGbWimiYEC7PUs8Bq8aAu9v8P8HAQ



NOTE: The Standard User Kit contains the minimal supplies necessary for a project. Your project may have more specific requirements, and you may need to order additional supplies.

To see a list of the supplies not included in the Standard User Kit, click here:

SHOW ITEMS NOT IN THE STANDARD USER KIT

NOTE: The Standard User Kit contains the minimal supplies necessary for a project. Your project may have more specific requirements, and you may need to order additional supplies.

NOTE: The Standard User Kit contains the minimal supplies necessary for a project. Your project may have more specific requirements, and you may need to order additional supplies.

These supplies are NOT included in the STANDARD USER KIT:

CP3 CRYOPLUNGE PLUNGING TWEEZER

http://www.emsdiasum.com/microscopy/products/grids/cflat.aspx#CP3690

Quantifoil, 200#, 2um, Copper

IF YOU PURCHASE A STANDARD USER KIT, IT WILL CONTAIN ONLY FOUR OF THESE. YOU WILL NEED A CONSTANT SUPPLY.

http://www.emsdiasum.com/microscopy/products/grids/quantifoil.aspx#SiO2

C-Flat Grids, 2um/2um-2C, Copper

IF YOU PURCHASE A STANDARD USER KIT, IT WILL CONTAIN ONLY FOUR OF THESE. YOU WILL NEED A CONSTANT SUPPLY.

http://www.emsdiasum.com/microscopy/products/grids/cflat.aspx#CF

If you have unclipped grids for an upcoming Krios session you are required to purchase one of two kits:

KRIOS CARTRIDGE KIT ($85.00)

The Krios Cartridge Kit contains the supplies sufficient for 1 clipping from SEMC. SEMC staff have assigned grid clipping days and supplies must be presented or paid for at that time for grids to be clipped and scheduled for upcoming Krios time. We DO accept pre-clipped grids in new AutoGrids, and also grids in new AutoGrids that have been previously imaged using a different Krios. However, we reserve the right to inspect these grids and reject them for loading based on their integrity. Used AutoGrids are NOT permitted because they invalidate our warranty and have the potential to result in many weeks of downtime for the instrument.

To see a list of the Krios Cartridge Kit supplies, click here:

To order supplies directly from FEI, you will have to contact them directly and arrange to order supplies:

FEI
5350 NE Dawson Creek Drive
Hillsboro, Oregon 97124
USA
Phone: +1 (503) 726-7500

These are the necessary supplies:

A cartridge is a C-clip plus C-clip ring:

  • Autogrid C-clip rings (100 pk) #1036173
  • Autogrid C-clips (100 pk) #1036171

An autogrid box holds 4 cartridges:Autogrid containers & lids (20 pk) #1084591

For your convenience, you may also purchase supplies sufficient for 1 clipping from SEMC.

The Krios Cartridge Kit contains:

  • 4x C-Clips
  • 4x AutoGrids
  • 1x AutoGrid Box

Price for the Krios Cartridge Kit: $85.00
(prices may change if the cost to us rises)

KRIOS CARTRIDGE MINI-KIT ($69.00)

The Krios Cartridge Mini-Kit contains all the supplies sufficient for 1 clipping from SEMC EXCEPT the AutoGrid Box. SEMC staff have assigned grid clipping days and supplies must be presented or paid for at that time for grids to be clipped and scheduled for upcoming Krios time. We DO accept pre-clipped grids in new AutoGrids, and also grids in new AutoGrids that have been previously imaged using a different Krios. However, we reserve the right to inspect these grids and reject them for loading based on their integrity. Used AutoGrids are NOT permitted because they invalidate our warranty and have the potential to result in many weeks of downtime for the instrument.

To see a list of the Krios Cartridge Mini-Kit supplies, click here:

To order supplies directly from FEI, you will have to contact them directly and arrange to order supplies:

FEI
5350 NE Dawson Creek Drive
Hillsboro, Oregon 97124
USA
Phone: +1 (503) 726-7500

These are the necessary supplies:

A cartridge is a C-clip plus C-clip ring:

  • Autogrid C-clip rings (100 pk) #1036173
  • Autogrid C-clips (100 pk) #103671

For your convenience, you may also purchase supplies sufficient for 1 clipping from SEMC.

The Krios Cartridge Mini-Kit contains:

  • 4x C-Clips
  • 4x AutoGrids

Price for the Krios Cartridge Mini-Kit: $69.00
(prices may change if the cost to us rises)

Payment can be made in one of two ways:

  1. Write a check payable to New York Structural Biology Center, Inc., give it to Ashleigh Raczkowski or Kelsey Jordan. Ashleigh and Kelsey can be found in the SEMC lab M-F, 9:00am – 4:00pm. PURCHASE YOUR SUPPLIES PRIOR TO YOUR LAB SESSION.
  2. Purchase your kit with a credit card using PayPal.
    GO TO WWW.PAYPAL.COM and click on “Send Money”. Follow the instructions and use your credit card to send money to: Paypalkits@nysbc.org. Please print your receipt and bring it with you to the lab as proof you have paid. PLEASE INCLUDE IN YOUR PAYPAL E-MAIL THE SPECIFIC KIT YOU ARE PURCHASING (i.e., “User Kit”, “Krios Cartridge Kit” or “Krios Cartridge Mini-Kit”). Give your printed receipt to the lab and give a copy to Kelsey Jordan or Ashleigh Raczkowski. Kelsey and Ashleigh are available in the SEMC lab M-F, 9:00am – 4:00pm.

PURCHASE YOUR SUPPLIES PRIOR TO YOUR LAB SESSION. BE SURE TO BRING YOUR RECEIPT WITH YOU TO YOUR SESSION.

Member Institutions