NYSBC - New York Structural Biology Center

News and Events

New Krios Microscopes
biotwinw The Krios#1 microscope has been operational despite the construction, and has been producing high quality data more or less continuously. The Krios#2 microscope is now in open beta. The Krios#3 is built, and is currently in a testing stage.

August 3rd, 2017 - 12:00pm
SEMC FORUM
A New Wavelet Denoising Method for Experimental Signals
Madhur Srivastava and Jack Freed, Cornell University

Madhur Srivastava and Jack H. Freed of ACERT (National Biomedical Center for Advanced ESR Technology) presented their new method to denoise experimental signals using wavelet transforms. To learn more about it and read their abstract, click here.

July 11th, 2017 - 9:30am - 4pm
nut New User Orientation

Although New User Orientation is offered on the first Tuesday of every month, July's session was held on the 11th to accommodate the Fourth of July holiday. The morning session covered covers the general laboratory policies and safety training, and the afternoon session includes a demonstration of TEM use and introduction to Leginon. To inquire about taking the next New User Orientation, please click here.

June 27th, 2017
APPION PART 2: 3D RECONSTRUCTION
NewUser

On June 27th, an Appion Part 2 Workshop was held in the SEMC Conference Room. Appion Part 2 is a follow-on to the Part 1 workshop and focuses on 3D initial models and refinement. To inquire when this workshop will be offered again, please e-mail EMG@NYSBC.org. Attendance is limited to 10 participants.

June 6th, 2017
New User Training


NewUser Attended by these people in the photo above, New User Training took place on June 6th. The morning session covers sample preparation (negative-staining and plunge freezing), and the afternoon session covers basic TEM use (loading samples and introduction to Leginon). Please email EMG@NYSBC.org to inquire about future training session dates.

May 30th, 2017
APPION PART 1: SINGLE-PARTICLE ANALYSIS - BASICS
app1b

The Appion Part 1 Workshop provides hands-on training for the Appion single-particle data processing workflow. Appion is a "pipeline" for processing and analysis of EM images; it is integrated with Leginon data acquisition, but can also be used stand-alone after uploading images or particle stacks. Topics include analyzing the quality of your data collection, correcting the CTF, generating and cleaning up stacks, and generating 2D class averages. To inquire about when the next Appion 1 workshop will be held, please email EMG@NYSBC.org.

An Appion Part 2 Workshop will be offered on June 27th, 2017 to provide training for making initial models and doing 3D reconstructions. To register for this workshop, click here: https://www.surveymonkey.com/r/8R6N9RB. Attendance is limited to 10 participants.

May 25th, 2017
HOLEY GOLD GRID WORKSHOP
Holy

This workshop provides an overview of making holey gold films from holey carbon films supported on gold mesh. Holey gold films provide a stronger support film and reduce beam induced movement of the specimen. Please email EMG@NYSBC.org to inquire about registering for the next Holey Gold Workshop.

May 18th, 2017
SEMC FORUM
Dragonfly, a Free but Valuable Platform for Image Visualization and Analysis
Mike Marsh, Object Research Systems

On May 18th, Mike Marsh of Object Research Systems gave a Forum on their Dragonfly software. Dragonfly is a commercially supported image processing and visualization platform for 2D/3D/4D microscopy and other imaging data. To read the abstract, please click here.

May 9, 2017
Appion Part 3 Workshop: Tomography

Leginon-Appion-Protomo: Semi-Automated Fiducial-less Tilt-Series Collection, Alignment, and Sub-Tomogram Processing
app3 On May 9th, Alex Noble and Julia Brasch gave a workshop that is part of the Appion Workshop Series.

Tomography performed without fiducials has historically been an unreliable and innacurate task. At NYSBC we have developed a full workflow for fiducial-less tilt-series collection and alignment. This workshop teaches you how to perform tomography on all sample types, from single particle samples to FIB/SEM lamella for visualization in situ and for further processing with sub-tomogram alignment and averaging.

Users learn the basics of tilt-series collection in Leginon, tilt-series alignment with Appion-Protomo, and sub-tomogram processing with Dynamo. Appion-Protomo and Dynamo is partially hands-on.

No previous experience in tomography is required. Workshop attendees are encouraged to bring data in the form of tilt-series images, tomograms, or sub-tomograms for processing assistance.

Attendees should bring their own laptop in order to follow along during this hands-on workshop. Please email EMG@NYSBC.org to inquire about future workshop dates.

May 2, 2017
New User Training


NewUser Attended by these people in the photo above, New User Training took place on May 2nd. The morning session covers sample preparation (negative-staining and plunge freezing), and the afternoon session covers basic TEM use (loading samples and introduction to Leginon). Please email EMG@NYSBC.org to inquire about future training session dates.

April 26, 2017
Workshop on Addressing Challenges in CryoEM Grid Preparation


cryo On April 26th, 2017 a workshop titled "Workshop on Addressing Challenges in CryoEM Grid Preparation" was presented in the SEMC conference room and was attended by those pictured above.

There are many challenges in preparing vitrified specimens of suitable ice thickness, and suitable particle preservation, distribution, and orientation, in a repeatable, reliable and robust manner. A major goal of the workshop was to understand the issues underlying these challenges and to discuss new approaches that will lead to improvements in all of these aspects.

April 18, 2017
Rosetta Workshop - Frank DiMaio


FDimaio On April 18th, 2017 a workshop titled "Workshop On Using Rosetta For CryoEM-Guided Protein Structure Determination" was presented by Frank DiMaio of the University of Washington in the Main Seminar Room at NYSBC. This tutorial introduced several Rosetta-based tools for structure determination guided by cryoEM density data. A general introduction to the Rosetta software suite was provided, and then specific methods for automated de novo structure determination, homology modeling, and model refinement were covered. The tutorial also described interpretation of Rosetta results and introduced several other useful tools in Rosetta to handle other experimental restraints.

April 4, 2017
New User Training


NewUser Attended by these people in the photo above, New User Training took place on April 4th. The morning session covers sample preparation (negative-staining and plunge freezing), and the afternoon session covers basic TEM use (loading samples and introduction to Leginon). Please email EMG@NYSBC.org to inquire about future training session dates.

March 22, 2017
SEMC Forum
Large-Scale Data with Nanoscale Insight
On March 22, Gabriella Kiss of FEI/Thermo Fisher Scientific presented a SEMC Forum titled Large-Scale Data with Nanoscale Insight in the Main Seminar Room, New York Structural Biology Center at 12:00pm. To learn more about this Forum, please click on "SEMC Forum" above.

March 21, 2017
Appion Part 2 Workshop


App2 Attended by the people you see in the photo above, a one-day Appion Part 2 Workshop was held on March 21st. This Workshop is a follow-on to the Part 1 workshop and focuses on 3D initial models and refinement. Please email EMG@NYSBC.org to inquire about future workshop dates.

March 7, 2017
New User Training


NewUser Attended by these people in the photo above, New User Training took place on February 21st. The morning session covers sample preparation (negative-staining and plunge freezing), and the afternoon session covers basic TEM use (loading samples and introduction to Leginon). Please email EMG@NYSBC.org to inquire about future training session dates.

February 23, 2017
Appion Workshop: Advanced Techniques - Going from Appion to RELION 2 and FREALIGN


Appadv A one-day master class was held February 23rd from 10:00AM to 4:00PM for experienced Appion users interested in using RELION2 and FREALIGN for 3D refinements. The focus of this workshop was how to take advantage of tools within Appion for efficient single particle analysis concurrent with Leginon data collection. A real-time demonstration was given using our GPU workstations including a brief overview of the RELION2 and FREALIGN interfaces. The workshop limited to 10 attendees. If you are interested in learning when the next Appion Advanced Workshop will be held, please e-mail EMG@NYSBC.org

February 6-7, 2017
Workshop on Management of Large CryoEM Facilities


Cryo Attended by the group pictured above, a Workshop on Management of Large CryoEM Facilities was held on February 6th and 7th. A major goal of the workshop was to discuss best practices for managing CryoEM facilities. Topics discussed included sample handling, instrument management, computational environments, training, user management and workflow. The agenda, PDFs of slides presented and a link to the YouTube recording is here: http://nramm.nysbc.org/27-282017-management-of-large-cryoem-facilities/.

February 2, 2017
New User Training


NewUser Although it is usually offered on the first Tuesday of every month, February's New User Training took place on February 2nd to accommodate workshops being offered next week. The morning session covers sample preparation (negative-staining and plunge freezing), and the afternoon session covers basic TEM use (loading samples and introduction to Leginon). Please email EMG@NYSBC.org to inquire about future training session dates. Pictured above are the most recent attendees.

Tuesday, 1/10/17
Appion Part 1 Workshop


App1 A one-day Appion Workshop was held on Tuesday, January 10th from 10:00AM to 4:00PM. The Appion Part 1 Workshop provides hands-on training for the Appion single-particle data processing workflow. Appion is a "pipeline" for processing and analysis of EM images and is integrated with Leginon data acquisition. Topics include analyzing the quality of your data collection, correcting the CTF, generating and cleaning up stacks, and generating 2D class averages. An Appion Part 2 Workshop will be offered in the following month to provide training for making initial models and doing 3D reconstructions. If you are interested in learning when the next Appion Part 1 Workshop will be held, please e-mail EMG@NYSBC.org.

Tuesday, 1/3/17
New User Training


NewUser Pictured here are the attendees of January's New User Training Session, offered the first Tuesday of every month. The morning session covers sample preparation (negative-staining and plunge freezing), and the afternoon session covers basic TEM use (loading samples and introduction to Leginon). Please email EMG@NYSBC.org to inquire about future training session dates.

Wednesday and Thursday 12/8-9
Dynamo Workshop

Dynamo Pictured above are the attendees of the Dynamo Workshop.

This workshop provides hands-on training in all practical aspects of sub-tomogram averaging using Dynamo. The new Dynamo catalogue system for organizing and centralizing tomograms will be featured along with new semi-automated picking procedures. All stages of the pipeline will be covered, including: tomogram visualization and archiving, particle picking, membrane picking, filament picking, averaging, refinement, and classification.

To learn when the Dynamo Workshop will be held again, please e-mail EMG@NYSBC.org.

Tuesday 12/7
Appion Part 3 Workshop

NewUser Pictured above are the attendees of the Appion Part 3 Workshop.

Tilt-series alignment is commonly performed by tracking fiducials in order to recover the three-dimensional structure and orientation of specimen in cryo-electron tomography, negative stain tomography, and tomography on plastic sections. However this approach is sometimes fraught with difficulty due to fiducial movement, fiducial aggregation, or the lack of fiducials in specimen such as FIB/SEM lamella.

In this workshop users gain hand-on experience with a semi-automated implementation of Protomo into Appion. Protomo allows for fiducial-less alignment of tilt-series and refinement of specimen orientation on the grid through an iterative geometry refinement process. Appion-Protomo, which installs seamlessly with Appion, significantly reduces the effort and expertice required by the user to align fiducial-free tilt-series collected using any software package. All that is required is a stack of tilt images along with knowledge of their imaging conditions. Integrated within Appion-Protomo are pipelines for defocus estimation, CTF correction, and dose compensation, along with multiple reconstruction methods.

To learn when the next Appion Part 3 Workshop will be held, please e-mail EMG@NYSBC.org.

Tuesday 12/6
New User Training

NewUser Pictured above are the attendees of the December New User Training, offered the first Tuesday of every month. The morning session covers sample preparation (negative-staining and plunge freezing), and the afternoon session covers basic TEM use (loading samples and introduction to Leginon). Please e-mail EMG@NYSBC.org to learn about future new user training sessions.

Friday, October 28th
Computational Methods for CryoEM Workshop

compu Pictured here are the attendees of the Computational Methods for CryoEM Workshop, led by Sjors Scheres. This workshop focused on computational approaches for cryoEM. The goal was to bridge between the computational science community in NYC and the cryoEM community.

Tuesday, October 25th
Relion Workshop with Sjors Scheres

Sjors This one-day workshop focused on the RELION software package and was led by its developer Sjors Scheres (MRC-LMB). The training involved a hands-on tutorial for cryo-EM single-particle analysis.

For details about RELION, click here.

Tuesday 10/4
New User Training

NewUser Pictured here are the attendees of the October's New User Training, offered the first Tuesday of every month. The morning session covers sample preparation (negative-staining and plunge freezing), and the afternoon session covers basic TEM use (loading samples and introduction to Leginon). Please email EMG@NYSBC.org to inquire about future training session dates.

September 21-22
Phase Plate Workshop

Phase FEI's volta phase plate has been installed on Krios#1. A SEMC Phase Plate Workshop was held on September 21-22 and led by Rado Danev, MPI Martinstried. The workshop included a demonstration of the optimal use of the device and processing of phase plate data.

Tuesday 9/6
New User Training

NewUser This month's New User Training was attended by (back row, l-r) Alex Noble, Judith Kribelbauer, Micah Rapp, (front row, l-r) Ed Eng (leader)Stephanie Siegmund, Juncheng Wang, led also by Ashleigh Raczkowski and Bill Rice (not pictured above). New users are required to attend a group new user training session before use of the facility. We offer these 1-day training sessions the beginning of each month from 9am to 4:30pm. Please email EMG@NYSBC.org to inquire about future training session dates.

Thursday 8/25
Holey Gold Grid Workshop
HolyGold The most recent Holey-Gold Grid Workshop was held on August 25th, 2016, from 1:00-3:00PM. Our Holey-gold grid workshop shows users how to make their own holey-gold grids from standard holey carbon gold grids. Please email EMG@NYSBC.org to inquire about future training session dates.

Wednesday 8/24
SEMC Forum: Prof. Jose-Maria Carazo, Spanish National Center for Biotechnology, Madrid, Spain
Cryo-EM data processing and information integration over the Web

Show/Hide Abstract

Thursday 8/18
SEMC Forum: Dr. Joel Mancuso, Ph.D., Dr. Mark Riccio, Ph.D., Carl Zeiss Microscopy, LLC
Non-invasive High Resolution 3D X-ray Microscopy for Life Sciences, Materials Science, and Engineering.

Show/Hide Abstract

Thursday 8/8
SEMC Forum: Dr. Andy Stewart, University of Limerick
Electron diffraction tomography, its many flavors, and applications to beam sensitive specimens.

Show/Hide Abstract

Tuesday 7/26
Appion Part 2 Workshop

Appion2 A one-day Appion Workshop was held on July 26, 2016 from 10:00AM to 4:00PM. This Appion Part 2 Workshop was a follow-on to the Part 1 workshop and focused on 3D initial models and refinement. If you are interested in attending an Appion Part 2 Workshop, please register at: https://www.surveymonkey.com/r/5NK7NGQ. The workshop is limited to 10 attendees.

Thursday 7/21
SEMC Forum: Myriam Badr, Ph.D.
Application Scientist
Nanotemper Technologies, Cambridge, MA
Exploring protein thermal and colloidal stability by NanoDSF

Show/Hide Abstract

Thursday 6/16
Holey Gold Grid Workshop

holeygold1 A workshop on making Holey Gold grids was held on Thursday, June 16 from 1:00 to 3:00pm. This provides an overview of making holey gold films from holey carbon films supported on gold mesh. Holey gold films provide a stronger support film and reduce beam induced movement of the specimen. If you would like to attend a holey gold workshop, please register at: https://www.surveymonkey.com/r/6M8ZT5V. The workshop is limited to 6 attendees.

Tuesday 6/14
Appion Part 1 Workshop

appion1 A one-day Appion Workshop was held on Tuesday, June 14, 2016 from 10:00AM to 4:00PM. The Appion Part 1 Workshop provides hands-on training for the Appion single-particle data processing workflow. Appion is a &ldq uo;pipeline” for processing and analysis of EM images; it is integrated with Leginon data acquisition, but can also be used stand-alone after uploading images or particle stacks. Topics include analyzing the quality of your data collection, correcting the CTF, generating and cleaning up stacks, and generating 2D class averages. If you are interested in attending an Appion Part 1 Workshop, please register at: https://www.surveymonkey.com/r/6TTP5PT. The workshop is limited to 10 attendees.

Monday 5/9
SEMC Forum: Professor Michael Elbaum
Department of Materials and Interfaces
Weismann Institute of Science
STEM: a re-emerging modality for cryo-imaging and tomography

Show/Hide Abstract

Tuesday, April 26th
SEMC Workshop: A hands-on workshop on three multi-scale visualization and modeling packages will be held on Tuesday, April 26 by Willy Wriggers, Julio Kovacs, and Manual Wahle. Situs is a script-based program package for the 3-D fitting of atomic resolution structures into low-resolution density maps, e.g., from electron microscopy, tomography, or small angle x-ray scattering. TimeScapes bridges between fast and slow time scales and generates 3-D spatial heat map images from a statistical analysis of 1-D time series (or equivalent independent variable) data. Sculptor is a multi-scale molecular graphics package that serves both as a frontend for Situs algorithms and as a standalone 3-D visualization program. The three packages are freely available at the Wriggers lab website and are also supported by the SBGrid consortium (sbgrid.org) in the upcoming release. Please register at:  https://www.surveymonkey.com/r/3MF3P99.  The workshop is limited to 15 attendees.

Tuesday, April 26th
SEMC Forum: Dr. Willy Wriggers of the Department of Mechanical and Aerospace Engineering at Old Dominion University will give a talk entitled "Multiscale Biomolecular Modeling in 4-D".
Time: 12:00PM, Location: A-11 seminar room at NYSBC

Monday, April 25th
SEMC EM Course: Fitting Atomic Models.
Time: 3:00PM, Location: CUNY Advanced Science Research Center Auditorium

Wednesday, April 20th
SEMC Forum: Dr. Carolyn Larabell of the Department of Anatomy at the University of California, San Francisco will speak on "Localizing Molecules in Cellular CT Scans".
Time: 3:00PM, Location: CUNY Advanced Science Research Center Auditorium

Monday, April 18th
SEMC EM Course: Tom Walz of Rockefeller University will give a lecture on "Validation Methods"
Time: 3:30-5:00PM. Location: A-11 seminar room at NYSBC from 3:30-5pm.

Friday, April 15th
SEMC Forum: Dr. Radoslaw Nowak will visit from Oxford University to speak about "X-Ray and Activity Fragment Screening Across Subfamilies of Lysine Demethylases".
Time: 12:00PM, Location : A-11 seminar room at NYSBC.

Tuesday, April 12th
Appion Training Workshop: The Appion Part 1 Workshop provides hands-on training for the Appion single-particle data processing workflow. Appion is a "pipeline" for processing and analysis of EM images; it is integrated with Leginon data acquisition, but can also be used stand-alone after uploading images or particle stacks. Topics include analyzing the quality of your data collection,correcting the CTF, generating and cleaning up stacks, and generating 2D class averages. The workshop is limited to 10 attendees. For more information and registration, click here: https://www.surveymonkey.com/r/FZLPDXF.  
Time: 10:00AM-4:00PM, Location: SEMC Conference Room at NYSBC

Monday, April 11th
SEMC EM Course: Class in 2D crystallography - Helical (part II)
Time: 3:30-5:00PM, Location: A-11 seminar room at NYSBC

Titan Krios

Titan Krios The Krios #1 microscope has been installed, accepted and is now being used to collect data for a first set of member projects. Images and a time lapse movie of the installation can be seen at http://semc.nysbc.org/krios.html. The microscope is currently equipped with a Falcon 2 camera that will be upgraded to the Falcon 3 camera later in the summer. The K2 camera will be moved from the JEM 2100 onto Krios #1 in April. Both the K2 and the Falcon 3 support counting mode, so users will have a choice of cameras for their projects.   We will be carrying out characterization tests on both cameras to help users identify the best match of cameras to their projects. A TVIPS F416 CMOS camera will replace the K2 on the JEM2100 and we expect that the JEM2100 will be an excellent cryo screening instrument.

We are delighted to announce that the Simons Foundation has generously provided support to purchase a second Krios microscope. This microscope will replace the JEM3200 and will be equipped with an energy filter, Cs corrector and a Volta phase plate. The JEM3200 will be decommissioned in April and the room will be prepared for expected delivery of Krios #2 in August. The DE20 camera that is on the JEM3200 will be transferred to the F20 microscope in April and we expect that the F20 will be capable of supporting projects for reconstructions of maps in the ~4A and above resolution range.

SEMC Renovations



A major renovation of the HVAC systems for the microscope suite will take place in the spring. The current HVAC system is not capable of maintaining a controlled temperature and humidity environment and this leads to instability in the instruments and impacts their performance. Work on the HVAC upgrade will start in May and we hope to have it all completed by July. Around the same time, work on the room for Krios #2 will also begin and be ongoing for a few weeks. This will include digging a pit in the concrete to accommodate an anti-vibration table. This work will inevitably at times cause some major disruptions in the control room and the microscopes may not be useable for some periods of time. Our intention is to keep the microscopes, especially the Krios, operational as much as possible during the entire period of construction.

FIM-SEM Mouse Rod OS

October 2015 New Publication: Architecture Formed by Large and Small Terminase Subunits Show/Hide Abstract





Titan Krios

Titan Krios SEMC is delighted to announce that due to the continued generosity of the Simons Foundation, we will soon install an FEI Titan Krios microscope with a Falcon 2 camera (shortly to be upgraded to a Falcon 3). We are currently renovating space to house the instrument, and we anticipate that it will be ready for users at the end of the first quarter of 2016. The Titan Krios/Falcon 3 instrument will join the current lineup of instrumentation, including the four other transmission electron microscopes (JEOL 3200FSC/DE20, JEOL 2100F/K2, JEOL 1230/CCD, Tecnai F20/CCD), and a dual beam focused ion beam scanning electron microscope (Helios 650).




Renovations

Renovations As many of you know, we completed the first round of renovations in May 2015. These changes provided our control room and conference room, (which are currently the hub of SEMC), as well as space for computational servers and many improvements to the electrical and computational infrastructure. We now need to begin a second round of renovations to create space for the new Titan Krios. The Krios will be installed in the room currently housing the Helios, and the Helios will be moved to a new space in the wet lab area. Work on this new space will begin very soon, and the move of the Helios is currently scheduled for the end of October. The instrument will be down during the move, and for a short time afterwards as it gets reinstalled and tested. The preparations for the Helios room will also mean that the dehumidified specimen preparation room will be unavailable for use starting next week for a few days. We will move the CP3 into the wet lab area during this time so that grids may still be vitrified. Once the Helios has been moved, the Krios room needs some major work in constructing a vibration isolation platform. This includes removal of concrete to construct a pit, which will inevitably cause disruption to all of the instruments, with the exception of the F20. We will do our best to minimize this disruption, but for some days it will not be possible to acquire data until the construction is complete. SEMC staff will work to adjust the schedule during this time to maximize the use of the instruments and try to best re-accomodate users who lose their booked sessions. Click here to view an updated Renovation schedule.




Training.png

New User Training New users are required to attend a group new user training session before use of the facility. We offer these 1-day training sessions the beginning of each month from 9am to 4:30pm. The morning session will cover sample preparation (negative-staining and plunge freezing), and the afternoon session covers basic TEM use (loading samples and introduction to leginon). New users may purchase a starter EM user kit from SEMC. New users are strongly encouraged to do some background reading before their first training session.

Following training users may request time on the on the instruments in consultation with SEMC staff until they gain sufficient experience to receive sign off from SEMC staff to use the instruments independently. Please note that each instrument requires individual training and sign off, which will be provided by SEMC staff on a one-on-one basis.

Please email EMG@NYSBC.org to inquire about future training session dates.




CryoEM

August 2015 On August 5th, 2015 BioTechniques published a piece on the revolution of CryoEM, courtesy of Kelly Chi. Mentioned in the post were Clint Potter and Bridget Carragher, co-directors of the Simons Electron Microscopy Center at the New York Structural Biology Center, and according to the article, it has been an exciting two years for CryoEM. From how CryoEM works, to the challenge of breaking the 3Å resolution barrier, to discussing why CryoEM is useful when x-ray crystallography also garners high resolution imaging, all the way to what lies ahead and the challenges the field is expected to take, this article gives an interesting, concise outlook on the field of Cryo Electron Microscopy.

Read the full text here.




FIM-SEM Mouse Rod OS

April 2015 New Publication: Mammalian Photoreceptors Ya-Chu Hsu, Jen-Zen Chuang, and Ching-Hwa Sung from Weil Medical College have published a paper concerning the mechanism through which rod photoreceptors in mammals maintain a balance between membrane bound discs that are created and discarded daily.




SEMC

March 2015: NYSBC Electron Microscopy staff assists users in evaluating the suitability of existing samples for achieving specified goals and in implementing any unusual experimental approaches. Staff members are also available for pilot studies to produce preliminary reconstructions suitable for inclusion in grant proposals. Staff is also available to collaborate with scientists who are not EM users but need EM data for their projects




2.8A

March 2015: New Publication: 2.8Å Resolution Proteasome Stucture Melody Campbell from the Scripps Research Institute and David Veesler from the University of Washington worked with Bridget Carragher, Clint Potter, and Anchi Cheng from the Electron Microscopy Group at NYSBC using single-particle cryoEM to produce images of protein structure detailed enough to be useful for drug development.