About the course
Electron microscopy in combination with image analysis is increasingly powerful in producing 3D structures of individual molecules and large macromolecular complexes that are unapproachable by other methods. This course is focused on the concepts and theories behind electron microscopy and will be taught in a reverse classroom format based on Grant Jensen’s online course (Getting Started in Cryo-EM from Caltech). Students will be responsible for watching these online lectures prior to class. Each week guest lecturers and SEMC staff lead discussions on the practice of solving molecular structures by electron microscopy.
The course will be held at the New York Structural Biology Center at 89 Convent Ave (133rd St).
Classes in SEMC seminar room (Mondays 3:30-5pm and select Wednesdays 3:30-5pm)
EM fundamentals section (month of January)
Lecture1 : Introduction & SEMC tour
Lecture2 : Basic anatomy of the electron microscope
Lecture3 : Challenges in biological EM & Sample Prep and practical
Lecture4 : Support films and practical
Lecture5: Fourier transforms and Image Formation
Lecture1: Tomography (part I: Intro and overview of ET)
Lecture2 : Tomography (part II: FIB-SEM)
Lecture3 : Tomography (part III: Cryo-Applications & Sub-tomogram averaging)
Single particle section
Lecture1: Single-particle analysis (part I: Intro and overview of SPA)
Lecture2 : Single-particle analysis (part II: Data Analysis and reconstruction workflow
Lecture3 : Single-particle analysis (part III: Reconstruction workflow (cont), and Interpretation and Limitations)
EM crystallography section
Lecture1 : MicroED and 2D crystallography intro
Lecture2 : Helical (part I: Intro and overview of helical)
Lecture3 : Helical (part II: Helical cont)
EM challenges and new frontiers
Lecture1 : Validation methods
Lecture2 : EMDataBank: Structure Data Archiving, Validation Challenges
Lecture3 : Moderate resolution interpretation
Lecture4 : Fitting Atomic Models
The lecture slides are provided for educational purposes only. All rights are reserved by their original authors. Please contact them if you would like to use or repost their content.
Practicals/Journal club in the SEMC conference room (starts @3:30pm)
Edward Eng (NYSBC, Manager): Course leader
Micah Rapp (COLU, Graduate student): Teaching assistant
For researchers taking the course for credit, the final score will be tabulated as follows:
Practical Worksheet 10% x 4
Suggested class structure (1.5 hr class)
45 min Introduction by guest lecturer on applying the class topic to scientific questions with focus on the nuts & bolts of the technique. Examples may be from their own research or recent papers.
30 min Open ended discussion on the topic. Conversation starters may come from concept check questions on the topic or presentation on newer developments in the field.