Please sign up here so we have an accurate headcount of students to book classrooms: https://www.surveymonkey.com/r/WinterCourse20 .
About the course
Electron microscopy in combination with image analysis is increasingly powerful in producing 3D structures of individual molecules and large macromolecular complexes that are unapproachable by other methods. This course is focused on the concepts and theories behind electron microscopy and will be taught in a reverse classroom format based on Grant Jensen’s online course (Getting Started in Cryo-EM from Caltech). Students will be responsible for watching these online lectures prior to class. Each week guest lecturers and SEMC staff lead discussions on the practice of solving molecular structures by electron microscopy.
The course will be held at the New York Structural Biology Center at 89 Convent Ave (133rd St).
Classes in SEMC seminar room (Mondays 3:30-5pm and select Wednesdays 3:30-5pm)
EM fundamentals section (month of January)
Jan 6 : Lecture – Introduction & SEMC tour
Jan 8 : Practical – Journal club
Jan 13 : Lecture – Basic anatomy of the electron microscope
Jan 15 : Practical – TEM use
Jan 20 : MLK Jr holiday – No class
Jan 22 : Practical – Sample Preparation & Support films
Jan 27 : Lecture – Fourier transforms and Image Formation
Jan 29 : Practical – Image pre-processing
Feb 3 : Lecture – MicroED
Feb 5 : Practical – Journal club
Feb 10: Lecture – Helical reconstruction
Feb 12 : Practical – Journal club and wrap up
Single particle section
See SPA short course here. Students that attend the full course will be allowed to participate in the morning lectures, but would have to apply to be admitted to the afternoon practicals.
See tomography short course here. Students that attend the full course will be allowed to participate in the morning lectures, but would have to apply to be admitted to the afternoon practicals.
The lecture slides are provided for educational purposes only. All rights are reserved by their original authors. Please contact them if you would like to use or repost their content.
Practicals/Journal club in the SEMC conference room (starts @3:30pm)
Edward Eng (NYSBC, Manager): Course leader
Micah Rapp (COLU, Graduate student): Teaching assistant
For researchers taking the course for credit, the final score will be tabulated as follows:
- Recitation 40%
- Practical Worksheet 10% x 4
- Attendance 20%
Suggested class structure (1.5 hr class)
- Introduction by a lecturer on applying the class topic to scientific questions with focus on the nuts & bolts of the technique. Examples may be from their own research or recent papers.
- Open ended discussion on the topic. Conversation starters may come from concept check questions on the topic or presentation on newer developments in the field.