The Winter-Spring 2023 EM Course

Sign up for the course here: https://www.surveymonkey.com/r/SEMC_course_Registration
If you are taking the course for credit make sure to register through your institution’s registrar as well. 

About the course

Electron microscopy in combination with image analysis is increasingly powerful in producing 3D structures of individual molecules and large macromolecular complexes that are unapproachable by other methods. This course is focused on the concepts and theories behind electron microscopy and will be taught in a reverse classroom format based on Grant Jensen’s online course (Getting Started in Cryo-EM from Caltech) and cryoEM101. Students will be responsible for watching these online lectures prior to class. Each week SEMC staff lead discussions on the practice of solving molecular structures by electron microscopy.

Class schedule

Classes in SEMC or NYSBC seminar rooms (Winter course: Mondays 3:30-5pm and Wednesdays 3:30-5pm | Short course weeks: 9:30am-11am)

EM fundamentals section

Jan 9 Lecture: Introduction & Basic anatomy of the electron microscope
Jan 11 Recitation: Roundtable on EM modalities
Jan 16 Martin Luther King Jr Holiday
Jan 18 Recitation: Support films and practical
Jan 23 Lecture: Challenges in biological EM & Sample Prep
Jan 25 Recitation: Negative stain and sample vitrification
Jan 30 Lecture: Image formation and data collection considerations
Feb 1 Recitation: TEM introduction

Single Particle Analysis

Feb 6 Lecture: Data collection and microscopy
Feb 8 Recitation: Grid screening and evaluation
Feb 13 Lecture: Image processing
Feb 15 Recitation: Image processing practical
Feb 20 President’s Day Holiday
Feb 22 Recitation: cryoEM proficiency certification [on your own]

EM challenges and new frontiers
Feb 27 Lecture: MicroED
Mar 1 Recitation: Journal club
Mar 6 Lecture: Validation methods
Mar 8 Recitation: Journal club
Mar 13 Lecture: Moderate resolution interpretation
Mar 15 Recitation: Journal club
Mar 20 Practical: Fitting atomic models
Mar 22 Recitation: Journal club
Mar 27 Lecture: Roundtable I
Mar 29 Recitation: Journal club
Apr 3 Lecture: Roundtable II
Apr 5 Recitation: Journal club

Tomography section

April 10-14 : Short course Keynote – Intro to Tomography

See the tomography short course here. Students that attend the full course will be allowed to participate in the morning lectures but would have to apply to be admitted to the afternoon practicals.

 

Class structure (1.5 hr class)

The course will be offered by the New York Structural Biology Center . Classes will be held Mondays 3:30-5pm and select Wednesdays 3:30-5pm. Times may be subject to change.

 

Previous courses:

Lecture slides from previous offerings may be found here: 2016, 2017, 20182019, 2020, 2021, and 2022 course.

The lecture slides are provided for educational purposes only. All rights are reserved by their original authors. Please contact them if you would like to use or repost their content.

 

Recitations:

Practicals/Journal club will be regularly scheduled over the semester to supplement the instruction. Typically on Wednesday @3:30pm.

 

Grading:

For researchers taking the course for credit, the final score will be tabulated as follows:

  • Recitation 40%
  • Practical Worksheet 10% x 4
  • Attendance 20%

 

To join the mailing list signup here:
https://www.surveymonkey.com/r/SEMC_course_Registration
Note: If you are taking the course for credit make sure to sign up through your institution’s registrar as well. 

  •  CUNY GC course BICM88900, class number 58654 – Special Topics in Biochemistry – 3 credits 
  •  Columbia BCHM G6400 – Cryo-Electron Microscopy – 4 credits
  • Course coordinator: Ed Eng (NYSBC)
  • Teaching assistant: Mahira Aragon (NYSBC)
2022-08-31T20:22:51+00:00September 8th, 2020|Courses|