New user orientation [monthly]:
Appion Workshop Series
Appion Workshop Part 1: 2D Analysis
Appion Workshop Part 2: Single Particle Analysis - 3D Reconstruction
Appion Workshop Part 3: Tomography
In this workshop users will gain hands-on experience with a semi-automated implementation of Protomo into Appion. Protomo allows for fiducial-less alignment of tilt-series and refinement of specimen orientation on the grid through an iterative geometry refinement process. Appion-Protomo, which installs seamlessly with Appion, significantly reduces the effort and expertice required by the user to align fiducial-free tilt-series collected using any software package. All that is required is a stack of tilt images along with knowledge of their imaging conditions. Integrated within Appion-Protomo are pipelines for defocus estimation, CTF correction, and dose compensation, along with multiple reconstruction methods.
No previous experience in tilt-series alignment processing is required. Workshop attendees are encouraged to bring data in the form of tilt-series images or stacks for processing assistance.
Attedees are encouraged to sign up for the Dynamo workshop to learn how to extract and process subtomograms from their reconstructed tomograms.
Attendees should bring their own laptop in order to follow along during this hands-on workshop.
If you are interested in attending an Appion Part 3 Workshop, please email EMG@NYSBC.org.
Holey Gold Grid Workshop:
EM course [yearly]:
External Training and Courses
50th EMBO Lecture Course
International School of Crystallography
https://crystalerice.org/2017/, Erice, Sicily, June 2-11, 2017
Three Dimensional Electron Microscopy
Gordon Research Conference
https://www.grc.org/programs.aspx?id=10866, Les Diablerets Conference Center, Les Diablerets, Switzerland, June 11-16, 2017
Microscience Microscopy Congress International Conference 2017
https://mmc-series.org.uk/conference, Manchester Central Convention Complex, Manchester, UK - 03-06 July 2017
Microscopy & Microanalysis 2017 Meeting
https://microscopy.org/MandM/2017/, American's Center, St. Louis, MO - 06-10 August 2017
24th Congress and General Assembly of the International Union of Crystallography
http://www.iucr2017.org/#, Hyderabad International Convention Centre, Hyderabad, India - August 21-28, 2017
Dynamo workshop 2017
http://nramm.nysbc.org/128-92017-dynamo-workshop, Biozentrum of the University of Basel, Basel, Switzerland, August 23-25
EMBO/EMBL Symposium: Seeing is Believing - Imaging the Processes of Life
https://www.embo-embl-symposia.org/symposia/2017/EES17-08/index.html, EMBL, Heidelberg, Germany, 4 October - 7 October 2017
X-Ray Methods & Structural Biology
https://meetings.cshl.edu/courses.aspx?course=C-CRYS&year=17, Cold Spring Harbor, NY, 16 October - 31 October 2017
To contact us for more information about one of our training courses, please email EMG@NYSBC.org.