Comparative Refinement as a Tool for Structural Validation

Tuesday 4/28
Stephen Murray
Baylor College of Medicine

The need for structural validation increases as electron cryomicroscopy structures go to a higher resolution. Comparative refinement utilizes the same data set that has already been collected to perform reconstructions in multiple software packages, and this can be used as a tool to perform the needed validation. When it is used in conjunction with other validation techniques, such as tilt-validation or class-average comparison, comparative refinement has the ability to show structural variability due to algorithmic bias. The greatest advantage of comparative refinement versus another validation technique, (e.g. tilt-validation), is that it requires no additional data collection. In this forum we will look at reconstructions of different specimens and examine what comparative refinement demonstrates.


2018-01-30T21:19:36+00:00April 28th, 2015|Forums|