INSTRUMENTATION2020-10-20T20:05:19+00:00

Instrumentation

Electron Microscopes

The microscopes are housed in specially built sound proof rooms, with each microscope placed on a concrete slab that extends to Manhattan bedrock or on a customized vibration table. This construction allows the instruments to be isolated from vibrations or other disturbances commonly found in the city. The walls are sand-filled to keep the rooms quiet. An air conditioning system with duct socks produces near laminar flow, and wall padding minimizes perturbations. The Krios and Helios rooms are additionally fitted with Electro-Magnetic isolation systems.

Transmission Electron Microscopes (TEMs)

FEI Titan Krios#1

  • Volta phase plates
  • Gatan K3 direct e- detector
    • 5,760 x 4,092
    • energy filter
  • Ceta CMOS detector 4k x 4k
  • Cs: 2.7 mm

FEI Titan Krios#2

  • Cs corrector
  • Volta phase plates
  • Falcon III direct e- detector 4k x 4k
  • Gatan K3 direct detector
    • 5,760 x 4,092
    • energy filter
  • Ceta CMOS detector 4k x 4k
  • Cs: 0.001 mm
  • 105,000X mag: 1.096 Å/pixel
  • 130,000X mag: 0.8549 Å/pixel
  • 165,000X mag: 0.65 Å/pixel

FEI Titan Krios#3

  • Volta phase plates
  • Falcon III direct e- detector 4k x 4k
  • Gatan K3 direct detector
    • 5,760 x 4,092
    • energy filter
  • Ceta CMOS detector 4k x 4k
  • Cs: 2.7 mm
  • 130,000X mag: 1.0605 Å/pixel (may be closer to 1.0475Å/px)
  • 165,000X mag: 0.8276 Å/pixel
  • 215,000X mag: 0.67 Å/pixel
Generation 3 Krios

Thermo Fisher – Titan Krios#4

  • Volta Phase Plates
  • Gatan K3 direct e- detector
    • 5,760 x 4,092
    • energy filter
  • Ceta CMOS detector 4k x 4k
  • Cs: 2.7 mm
Generation 3 Krios

Thermo Fisher –  Titan Krios#5

  • Gatan K3 direct e- detector
    • 5,760 x 4,092
    • energy filter
  • Ceta CMOS detector 4k x 4k
  • Cs: 2.7 mm
  • 81,000X mag: 1.069 Å/pixel
  • 105,000X mag: 0.825 Å/pixel
  • 130,000X mag: 0.67 Å/pixel
Generation 3 Krios

Thermo Fisher –  Titan Krios#6

  • Gatan K3 direct e- detector
    • 5,760 x 4,092
    • energy filter
  • Ceta CMOS detector 4k x 4k
  • Cs: 2.7 mm
  • 64,000X mag, native super-res: 0.686 Å/pixel
  • 105,000X mag: 0.889 Å/pixel
  • 130,000X mag: 0.661 Å/pixel
Generation 4 Krios

Thermo Fisher –  Titan Krios#7

  • 80-300 kV
  • Ceta CMOS detector 4k x 4k
  • Falcon IV direct e- detector
  • 250fps – 4096 x 4096 pixels
  • Automated and contamination-free loading of cassettes, containing up to 12 Autogrids
  • Cs: 2.7 mm

Tecnai T12 BioTWIN

  • TVIPS F416 CMOS detector 4k x 4k
Hitachi 7800

Hitachi 7800

  • Screening microscope
  • TVIPS F416 CMOS detector 4k x 4k

JEOL JEM-1230

  • Gatan US-4000 CCD detector 4k x 4k

Thermo Scientific Glacios

  • 80-200 kV
  • Ceta CMOS detector 4k x 4k
  • Falcon III direct detector 4k x 4k
  • Automated and contamination-free loading of cassettes, containing up to 12 Autogrids

FEI Tecnai F20

  • DE20 direct detector 4k x 5k
  • TVIPS  F416 CMOS detector 4k x 4k
  • Gatan Elsa holder for overnight operation
  • Cs: 2.0 mm
  • DE20: 29,000X mag: 1.54 Å/pixel
  • DE20 : 50,000X mag: 0.87 Å/pixel

Focused Ion Beam – Scanning Electron Microscope (FIB-SEM)

Scanning Electron Microscope (SEM)

Light Microscope

FEI Helios 650

TLD, ETD, ICE detectors

EMCrafts Cube II

Tabletop SEM

Nikon Eclipse Ti U

Epi-Fluorescence Module

Sample Prep

Chameleon

Sample vitrification instrument

ZEISS LSM 900 with Airyscan 2

Compact Confocal for Multiplex Imaging

Gatan Solarus II

Plasma Cleaner

Leica EM UC6

Ultramicrotome

Computational Resources

1 PB GPFS
Parallel File System

Additional equipment:

  • Plunge Freezers: Gatan CP3 Cryoplunge 3, FEI Vitrobot, Leica EM GP2
  • High Pressure Freezers: Bal-Tec HPM 010 High Pressure Freezing Machine, Wohlwend HPF Compact 01
  • Evaporator: Cressington Carbon Evaporator
  • Mass Photometer: Refeyn One(MP)