Instrumentation
Electron Microscopes
The microscopes are housed in specially built sound proof rooms, with each microscope placed on a concrete slab that extends to Manhattan bedrock or on a customized vibration table. This construction allows the instruments to be isolated from vibrations or other disturbances commonly found in the city. The walls are sand-filled to keep the rooms quiet. An air conditioning system with duct socks produces near laminar flow, and wall padding minimizes perturbations. The Krios and Helios rooms are additionally fitted with Electro-Magnetic isolation systems.
Transmission Electron Microscopes (TEMs)
TFS Titan Krios #1 “Jim”
Voltage: 300 kV
Cs: 2.7 mm
Volta Phase Plate (VPP)
Autoloader System
Gatan K3 Direct Electron Detector
5,760 x 4,092 pixels
BioQuantum Imaging Filter
Ceta CMOS Detector
4k x 4k pixels
64,000x mag: 1.3 Å/pixel
81,000x mag: 1.1 Å/pixel
105,000x mag: 0.8 Å/pixel
TFS Titan Krios #2 “Marilyn”
Voltage: 300 kV
Cs: 0.001 mm
Volta Phase Plate (VPP)
Autoloader System
Cs Corrector
Gatan K3 Direct Electron Detector
5,760 x 4,092 pixels
BioQuantum Imaging Filter
Ceta CMOS Detector
4k x 4k pixels
64,000x mag: 1.1 Å/pixel
81,000x mag: 0.8 Å/pixel
105,000x mag: 0.7 Å/pixel
TFS Titan Krios #3 “Audrey”
Voltage: 300 kV
Cs: 2.7 mm
Volta Phase Plate (VPP)
Autoloader System
Falcon III Direct Electron Detector
4k x 4k pixels
Gatan K3 Direct Electron Detector
5,760 x 4,092 pixels
BioQuantum Imaging Filter
Ceta CMOS Detector
4k x 4k pixels
64,000x mag: 1.3 Å/pixel
81,000x mag: 1.1 Å/pixel
105,000x mag: 0.8 Å/pixel
TFS Titan Krios #4 “Elizabeth”
Voltage: 300 kV
Cs: 2.7 mm
Volta Phase Plate (VPP)
Autoloader System
Gatan K3 Direct Electron Detector
5,760 x 4,092 pixels
BioQuantum Imaging Filter
Ceta CMOS Detector
4k x 4k pixels
64,000x mag: 1.3 Å/pixel
81,000x mag: 1.1 Å/pixel
105,000x mag: 0.8 Å/pixel
TFS Titan Krios #5 “Nathaniel”
Voltage: 300 kV
Cs: 2.7 mm
Volta Phase Plate (VPP)
Autoloader System
Gatan K3 Direct Electron Detector
5,760 x 4,092 pixels
BioQuantum Imaging Filter
Ceta CMOS Detector
4k x 4k pixels
81,000x mag: 1.069 Å/pixel
105,000x mag: 0.825 Å/pixel
130,000x mag: 0.67 Å/pixel
TFS Titan Krios #6 “Caitlin”
Voltage: 300 kV
Cs: 2.7 mm
Volta Phase Plate (VPP)
Autoloader System
Gatan K3 Direct Electron Detector
5,760 x 4,092 pixels
BioQuantum Imaging Filter
Ceta CMOS Detector
4k x 4k pixels
64,000x mag, native super-res: 0.686 Å/pixel
105,000x mag: 0.889 Å/pixel
130,000x mag: 0.661 Å/pixel
TFS Titan Krios #7 “Matthew”
Voltage: 300 kV
Cs: 2.7 mm
Volta Phase Plate (VPP)
Autoloader System
Falcon IV Direct Electron Detector
4092 x 4092 pixels
Ceta CMOS Detector
4k x 4k pixels
TFS Tecnai TF20
Voltage: 200 kV
Cs: 2.1 mm
Gatan Elsa™ Cryo-Transfer Holder
Gatan 626 Cryo-Holder
DE20 Direct Detector
4k x 5k pixels
TVIPS F416 CMOS Detector
4k x 4k pixels
DE20
29,000x mag: 1.54 Å/pixel
50,000x mag: 0.87 Å/pixel
Focused Ion Beam – Scanning Electron Microscope (FIB-SEM)
Dualbeam Cryo FIB-SEM Microscope
Scanning Electron Microscope (SEM)
Light Microscope
TFS Helios NanoLab 650
Landing Voltage Range
E-beam: 50 V – 30 kV
I-beam: 500 V – 30 kV
Probe Current
E-beam: 0.6 pA up to 26 nA
I-beam: 1 pA – 65 nA (15 position aperture strip)
Cryo Stage
Leica EM VCM Vacuum Cryo Manipulation System
Leica EM ACE600 High Vacuum Coater with Leica EM VCT500
Leica EM VCT500 Vacuum Cryo Transfer System
Elstar in-lens SE detector (TLD-SE)
Elstar in-lens BSE detector (TLD-BSE)
Everhart-Thornley SE detector (ETD)
IR camera for viewing sample/column
Door-mounted NavCam
SE and SI (secondary ion) detector (ICE)
Directional backscattered detector (DBS)
STEM detector with BF/DF/ HAADF segments
Integrated beam current measurement
TFS Aquilos 2 Cryo-FIB DualBeam
Electron Optics
Beam current: 1.5 pA to 400 nA
Accelerating voltage: 200 V – 30 kV
Resolution (with cryo-stage): 1.6 nm at 30 kV
2.6 nm at 2 kV (RT)
6.0 nm at 2kV (Cryo)
Ion Optics
Voltage: 500 V to 30 kV
Beam current: 1.5 pA – 65 nA in 15 steps
Resolution (with cryo-stage): 7.0 nm at 30 kV
TFS AutoTEM™ Cryo Software
TFS EasyLift™ NanoManipulator cryo-lift-out system
TFS Auto Slice and View™ Software (AS&V)
In-lens detection system: segmented lower (T1)
and upper detector (T2)
Everhart-Thornley SE detector (ETD)
IR camera for viewing sample/column
In-chamber TFS Nav-Cam™ Camera
Nikon Eclipse Ti U
Epi-Fluorescence Module
Sample Prep
Chameleon
Sample vitrification instrument
ZEISS LSM 900 with Airyscan 2
Compact Confocal for Multiplex Imaging
Gatan Solarus II
Plasma Cleaner
Leica EM UC6
Ultramicrotome
Computational Resources
1 PB GPFS
Parallel File System
Additional equipment:
- Plunge Freezers: TFS Vitrobot, Leica EM GP2
- High Pressure Freezers: Wohlwend HPF Compact 01
- Evaporator: Safematic high compact vacuum coating system
- Mass Photometer: Refeyn One(MP)
- Micropatterning system: Alveole Lab PRIMO
- Inverted Light Microscop: Leica DMi8