The Winter-Spring 2022 EM Course

To reseve a spot signup here:
If you are taking the course for credit make sure to sign up through your institution’s registrar as well.

 CUNY GC course BICM88900, class number 58654 – Special Topics in Biochemistry – 3 credits 
 Columbia BCHM G6400 – Cryo-Electron Microscopy – 4 credits

About the course

Electron microscopy in combination with image analysis is increasingly powerful in producing 3D structures of individual molecules and large macromolecular complexes that are unapproachable by other methods. This course is focused on the concepts and theories behind electron microscopy. Each week guest lecturers and SEMC staff lead discussions on the practice of solving molecular structures by electron microscopy. Students will be responsible for watching Grant Jensen’s online course (Getting Started in Cryo-EM from Caltech) ahead of attending the lectures. 

The course will be held at the New York Structural Biology Center at 89 Convent Ave (133rd St).

Course Schedule

Classes in SEMC seminar room (Mondays 3:30-5pm and select Wednesdays 3:30-5pm)

EM fundamentals section 
1/10/22 Lecture – Introduction & Basic anatomy of the electron microscope (SEMC staff)
1/12/22 Practical – TEM use (SEMC staff)
1/17/22 MLK Jr holiday – No class
1/19/22 Practical – Sample Preparation & Support films (SEMC staff)
1/24/22 Lecture – New cryoEM hardware and supporting a facility (SEMC staff)
1/26/22 Practical – Journal club

EM crystallography section
1/31/22 Lecture – MicroED (Bill Rice – NYU)
2/2/22 Practical – Journal club
2/7/22 Lecture – Helical reconstruction (Hernando Sosa – Einstein)
2/9/22 Practical – Journal club

Tomography section
2/14/22 Lecture – Tomography (Wei Dai — Rutgers)
2/16/22 Practical – Tomography workshop – Appion/Protomo (Alex Noble — NYSBC/SEMC)
2/21/22 President’s day holiday – No class
2/23/22 Practical – Journal club
2/28/22 Lecture – FIB-SEM (Bill Rice – NYU & SEMC staff)

Classes in the NYSBC seminar room (mornings)

Single-particle section* 
3/14/22 : Short course Keynote – Intro to Single Particle

*3/14-18/22 with instructors:

  • Joachim Frank (Columbia University)
  • Victor Chen & Wen Jian (Purdue University)
  • Fred Sigworth (Yale University)
  • Amedee des Georges & Reza Khayat (ASRC/City University of New York)
  • Rich Hite (Memorial Sloan Kettering Cancer Center)
  • Tom Walz (Rockefeller University)
  • Cathy Lawson (Rutgers University)
  • Oli Clarke (Columbia University)
  • Gira Bhabha & Damian Ekiert (New York University)

The single particle section will be a 1-week short course. See more information on the SPA short course here. Students that attend the full course will be allowed to participate in the morning lectures, but would have to apply to be admitted to the afternoon practicals.


Previous courses:

Lecture slides from the 2016 course may be found here, 2017 course may be found here, 2018 course may be found here, 2019 course may be found here, the 2020 course may be found here and 2021 course may be found here.

The lecture slides are provided for educational purposes only. All rights are reserved by their original authors. Please contact them if you would like to use or repost their content.


Practicals/Journal club in the SEMC conference room (starts @3:30pm)

Course instructors:

Edward Eng (NYSBC, Manager): Course leader

Mahira Aragon (NYSBC): Teaching assistant


For researchers taking the course for credit, the final score will be tabulated as follows:

  • Recitation 40%
  • Practical Worksheet 10% x 4
  • Attendance 20%

Suggested class structure (1.5 hr class)

  • Introduction by guest lecturer on applying the class topic to scientific questions with focus on the nuts & bolts of the technique. Examples may be from their own research or recent papers.
  • Open ended discussion on the topic. Conversation starters may come from concept check questions on the topic or presentation on newer developments in the field.



2021-11-19T14:20:29+00:00August 24th, 2020|Courses|