NYSBC - New York Structural Biology Center

Simons Electron Microscopy Center

About the Simons Electron Microscopy Center

The Simons Electron Microscopy Center provides expertise and resources for understanding both molecular and cellular structures. Molecular structure determination is enabled by high-end transmission electron microscopes (TEMs), direct detection cameras, and computational support for single particle analysis. Cellular structure determination is enabled by tomographic reconstructions using the TEMs and a focused ion beam (FIB) scanning electron microscope (SEM). SEMC is supported by dues from member institutions as well as the Simons Foundation.

News and Events

New Publication: Architecture Formed by Large and Small Terminase Subunits Bacteriophage
Packaging of viral genomes inside empty procapsids is driven by a powerful ATP-hydrolyzing motor, formed in many double-stranded DNA viruses by a complex of a small terminase (S-terminase) subunit and a large terminase (L-terminase) subunit, transiently docked at the portal vertex during genome packaging. Despite recent progress in elucidating the structure of individual terminase subunits and their domains, little is known about the architecture of an assembled terminase complex.

Click here to read the full text

New Instrumentation: The Titan KriosTitan Krios
SEMC is delighted to announce that due to the continued generosity of the Simons Foundation, we will soon install an FEI Titan Krios microscope with a Falcon 2 camera (shortly to be upgraded to a Falcon 3). We are currently renovating space to house the instrument, and we anticipate that it will be ready for users at the end of the first quarter of 2016. The Titan Krios/Falcon 3 instrument will join the current lineup of instrumentation, including the four other transmission electron microscopes (JEOL 3200FSC/DE20, JEOL 2100F/K2, JEOL 1230/CCD, Tecnai F20/CCD), and a dual beam focused ion beam scanning electron microscope (Helios 650).

SEMC Renovations Renovations
As many of you know, we completed the first round of renovations in May 2015. These changes provided our conference room and control room (which is the hub of SEMC), as well as space for computational servers and many improvements to the electrical and computational infrastructure. We are now in the middle of a second round of renovations to create space for the new Titan Krios. The Krios will be installed in the room that used to house the Helios, and the Helios has been moved to a new room off the wet lab area. The Helios is being tested and calibrated in its new space and will be available for use as of 11/18 for limited application*; Please email EMG@NYSBC.org for availability.. The Krios room is currently undergoing major reconstruction to incorporate a vibration isolation platform. This involves removal of concrete to construct a pit, which has meant that we have had to turn off the HVAC units to prevent concrete dust infiltration We have also sealed up the other three microscope rooms to keep them as clean as possible during this phase. We hope that the instruments will be available for use again by the beginning on the week of November 16. SEMC staff is working to adjust the schedule during this time to maximize the use of the instruments and will try to best re-accomodate users who have lost or will lose their booked sessions.

Click here to view an updated Renovation schedule.

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Thursday 11/19
Jack Johnson
Elden R. Strahm Emeritus Professor of Structural Virology

Ghost protocol: Seeing the invisible with cryoEM variance maps.

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Wednesday 9/16
Alex Noble
Florida State University

Automated Fiducial-less Tilt-Series Alignment with Protomo.
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