NYSBC - New York Structural Biology Center

Simons Electron Microscopy Center

The Simons Electron Microscopy Center provides expertise and resources for understanding both molecular and cellular structures. Molecular structure determination is enabled by high-end transmission electron microscopes (TEMs), direct detection cameras, and computational support for single particle analysis. Cellular structure determination is enabled by tomographic reconstructions using the TEMs and a focused ion beam (FIB) scanning electron microscope (SEM). SEMC is supported by dues from member institutions as well as the Simons Foundation.


SEMC Renovations Krios#2

Order in the control room is rapidly being restored.

Installing the Krios#2!

Members of the lab assist in positioning the base of the new Krios#2 in its case.


Installation of the BioTWIN is proceeding ahead of schedule.

Renovations to the SEMC suite are nearly complete. The Krios#1 FEI Falcon II has been upgraded to the FEI Falcon III direct detector. NYSBC is the first site in the US that has had this upgrade completed. Over the next semester the Falcon III will undergo beta-testing and integration with our Leginon automated data acquisition software system. We have also installed a new Volta Phase Plate on the Krios#1, and this is currently undergoing testing and characterization.

The BioTWIN screening microscope has arrived, and installation is nearly complete. The addition of this instrument allows greater throughput for optimization of vitrified samples.

The Krios#2 has arrived and is in the process of being installed. Krios#2 will have a Cs corrector, a phase plate, and an energy filter. The instrument will be suitable for both single particle and electron tomography projects.

The Krios#3 room is ready, as we expect the instrument to arrive in early December.

Until the latest construction phase is fully completed, please continue to email EMG@NYSBC.org to schedule microscope use.

We look forward to the completion of this long but necessary renovation project, and look forward to seeing many of you back in our warm and friendly control room again soon.

Click here to view an updated renovation schedule.
Click here to learn about scheduling microscope time during the renovation period.


Laboratory Supplies
There is new information on our "To Start" page regarding how to obtain the consumable laboratory supplies that users are required provide for their projects. To learn more about it, click here.

Staff LKim

Laura Kim has joined NYSBC as part of the SEMC operations staff. Laura is trained in molecular biology, biochemistry and negative-stain & cryo-EM, in the study of membrane and filamentous proteins. She is proficient in the use of Leginon/Appion and image processing for iterative helical real-space reconstruction. She joins our team from Gregory Alushin's lab at the National Institutes of Health, National Heart, Lung, and Blood Institute in Bethesda, MD and has formerly worked with Andreas Engel at Case Western Reserve University in Cleveland, OH and Ingeborg Schmidt-Krey at the Georgia Institute of Technology in Atlanta, GA.

Friday, October 28th, 9:00am
Computational Methods for CryoEM Workshop
This workshop will focus on computational approaches for cryoEM. The goal is to bridge between the computational science community in NYC and the cryoEM community.

For registration, visit: https://www.surveymonkey.com/r/QSX6VGH. There will be a limit of 15 attendees.

Tuesday, October 25th, 9:30am RELION Workshop with Sjors Scheres Sjors

This one-day workshop will focus on the RELION software package and is led by its developer Sjors Scheres (MRC-LMB). The training will involve a hands-on tutorial for cryo-EM single-particle analysis.

For details about RELION, click here.

October 4, 2016
New User Training

Pictured here are the attendees of the October's New User Training, offered the first Tuesday of every month. The morning session covers sample preparation (negative-staining and plunge freezing), and the afternoon session covers basic TEM use (loading samples and introduction to Leginon). Please e-mail emg@nysbc.org to learn about future new user training sessions.

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