NYSBC - New York Structural Biology Center

Simons Electron Microscopy Center

SEMC LogoAbout the Simons Electron Microscopy Center

The Simons Electron Microscopy Center provides expertise and resources for understanding both molecular and cellular structures. Molecular structure determination is enabled by high-end transmission electron microscopes (TEMs), direct detection cameras, and computational support for single particle analysis. Cellular structure determination is enabled by tomographic reconstructions using the TEMs and a focused ion beam (FIB) scanning electron microscope (SEM).

News and Events

New Article: The Climb of Cryo-EM CryoEM
On August 5th, 2015 BioTechniques published a piece on the revolution of CryoEM, courtesy of Kelly Chi. Mentioned in the post were Clint Potter and Bridget Carragher, co-directors of the Simons Electron Microscopy Center at the New York Structural Biology Center, and according to the article, it has been an exciting two years for CryoEM. From how CryoEM works, to the challenge of breaking the 3Å resolution barrier, to discussing why CryoEM is useful when x-ray crystallography also garners high resolution imaging, all the way to what lies ahead and the challenges the field is expected to take, this article gives an interesting, concise outlook on the field of Cryo Electron Microscopy.

Read the full text here.

New Publication: 2.8A Resolution Proteasome Structure 2.8A
Melody Campbell from the Scripps Research Institute and David Veesler from the University of Washington worked with Bridget Carragher, Clint Potter, and Anchi Cheng from the Simons Electron Microscopy Center using single-particle cryoEM to produce images of protein structure detailed enough to be useful for drug development.

For the full text, click here.

New Publication: Mammalian Photoreceptors FIM-SEM Mouse Rod OS
Ya-Chun Hsu, Jen-Zen Chuang, and Ching-Hwa Sung from Weil Medical College have published a paper concerning hte mechanism through which rod photoreceptors in mammals maintain a balance between memgrane bound discs that are created and discarded daily.

For the full text, click here.

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Thursday 11/19/2015
Jack Johnson
Elden R. Strahm Emeritus Professor of Structural Virology

Ghost protocol: Seeing the invisible with cryoEM variance maps.

Wednesday 9/16
Alex Noble
Florida State University

Automated Fiducial-less Tilt-Series Alignment with Protomo.


To see a list of past forum topics, please click here.

Before scheduling equipment or microscope time please email Bill or Ed for availability.

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Helios FIB-SEM
2.8A Proteosome

Microscopes and Detectors

  • JEM3200 FSC (300 keV, FEG), Direct Electron DE20
  • JEM2100 (200 keV, FEG), Gatan K2 Summit DD
  • JEM1230 (120 keV); Gatan US4000 CCD
  • Tecnai F20 (200keV, FEG); TVIPS CMOS CCD
  • Dual beam Scanning FIB/SEM


  • Single particle reconstruction
  • Electron tomography
  • Large scale serial reconstructions
  • In situ FIB milling plus tomography

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