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Simons Electron Microscopy Center

SEMC LogoAbout the Simons Electron Microscopy Center

The Simons Electron Microscopy Center provides expertise and resources for understanding both molecular and cellular structures. Molecular structure determination is enabled by high-end transmission electron microscopes (TEMs), direct detection cameras, and computational support for single particle analysis. Cellular structure determination is enabled by tomographic reconstructions using the TEMs and a focused ion beam (FIB) scanning electron microscope (SEM).

News and Events

New Equipment: Direct Detectors TEM Detector
Two new direct detectors have been intalled at the Simons Electron Microscopy Center. A Gatan K2 Summithas been installed on the JEOL 2100, and a Direct Electron DE20 has been installed on the JEOL 3200. These cameras are currently undergoing initial beta testing, but will be available for general use very soon.
New Publication: 2.8A Resolution Proteasome Structure 2.8A
Melody Campbell from the Scripps Research Institute and David Veesler from the University of Washington worked with Bridget Carragher, Clint Potter, and Anchi Cheng from the Simons Electron Microscopy Center using single-particle cryoEM to produce images of protein structure detailed enough to be useful for drug development.
New Publication Mammalian Photoreceptors FIM-SEM Mouse Rod OS
Ya-Chun Hsu, Jen-Zen Chuang, and Ching-Hwa Sung from Weil Medical College have published a paper concerning hte mechanism through which rod photoreceptors in mammals maintain a balance between memgrane bound discs that are created and discarded daily.

SEMC Forums:

Wednesday 6/17
Dipali G. Sashital
Iowa State University

Visualizing Conformational and Compositional Heterogeneity by Single-Particle EM

Friday 6/5
Dr. Rouslan Efremov
Vlaams Instituut voor Biotechnologie (Flanders Institute for Biotechnology)

Structural Insight into Calcium Signalling in Muscular Contraction


Before scheduling equipment or microscope time please email Bill or Ed for availability.

Helios FIB-SEM
CysZ Protein

Microscopes and Detectors

  • JEM3200 FSC (300 keV, FEG), Direct Electron DE20
  • JEM2100 (200 keV, FEG), Gatan K2 Summit DD
  • JEM1230 (120 keV); Gatan US4000 CCD
  • Tecnai F20 (200keV, FEG); TVIPS CMOS CCD
  • Dual beam Scanning FIB/SEM


  • Single particle reconstruction
  • Electron tomography
  • Large scale serial reconstructions
  • In situ FIB milling plus tomography