NYSBC - New York Structural Biology Center

Simons Electron Microscopy Center

The Simons Electron Microscopy Center provides expertise and resources for understanding both molecular and cellular structures. Molecular structure determination is enabled by high-end transmission electron microscopes (TEMs), direct detection cameras, and computational support for single particle analysis. Cellular structure determination is enabled by tomographic reconstructions using the TEMs and a focused ion beam (FIB) scanning electron microscope (SEM). SEMC is supported by dues from member institutions as well as the Simons Foundation.


SEMC Renovations Renovations

Major renovations to the SEMC suite will continue through most of July. Our current plan for the renovations is as below, but please bear with us as major changes like these cannot always be held to a strict timeline.

The JEOL JEM3200 has been decommissioned to make way for the new Krios that will arrive in Q3 this year. The room is now being renovated for the new instrument, which includes digging a pit in the concrete to accommodate the anti-vibration table. This is a very dirty and disruptive phase of the renovation project involving dust, noise, and vibration. We will shortly begin another disruptive phase to completely replace the HVAC system with one that will be able to maintain a stable environment for all the instruments in the suite. Unfortunately, this means that the JEM2100 and JEM1230 will go down as we cannot maintain cooling to the suite while the new duct work is installed. The JEM1230 has been moved to another location in the building (next to the FEI Tecnai F20) and we will get this back up and running as soon as a new water chiller arrives. We will try and keep the Krios operating by using a temporary HVAC system that will be located in the control room.

We will do our best to keep as many instruments up and functional as we can but we are certainly very much looking forward to the end of July when we believe that the suite will be returning to a more normal and functional state.

Click here to view an updated renovation schedule.
Click here to to learn about scheduling microscope time during the renovation period.


Appion Part 1 Workshop

A one-day Appion Workshop will be held on June 14, 2016 from 10:00AM to 4:00PM. The Appion Part 1 Workshop provides hands-on training for the Appion single-particle data processing workflow. Appion is a “pipeline” for processing and analysis of EM images; it is integrated with Leginon data acquisition, but can also be used stand-alone after uploading images or particle stacks. Topics include analyzing the quality of your data collection, correcting the CTF, generating and cleaning up stacks, and generating 2D class averages. If you are interested in attending an Appion Part 1 Workshop, please register at: https://www.surveymonkey.com/r/6TTP5PT. The workshop is limited to 10 attendees.

Holey Gold Grid Workshop

A workshop on making Holey Gold grids will be held on Thursday, June 16 from 1:00 to 3:00pm. This workshop will provide an overview of making holey gold films from holey carbon films supported on gold mesh. Holey gold films provide a stronger support film and reduce beam induced movement of the specimen. If you would like to attend this workshop, please register at: https://www.surveymonkey.com/r/6M8ZT5V. The workshop is limited to 6 attendees.

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