Simons Electron Microscopy Center
The Simons Electron Microscopy Center provides expertise and resources for understanding both molecular and cellular structures. Molecular structure determination is enabled by high-end transmission electron microscopes (TEMs), direct detection cameras, and computational support for single particle analysis. Cellular structure determination is enabled by tomographic reconstructions using the TEMs and a focused ion beam (FIB) scanning electron microscope (SEM). SEMC is supported by dues from member institutions as well as the Simons Foundation.
The Krios#1 FEI Falcon II has been upgraded to the FEI Falcon III direct detector. NYSBC is the first site in the US that has had this upgrade completed. Over the next semester the Falcon III will undergo beta-testing and integration with our Leginon automated data acquisition software system.
In October we are preparing for the arrival of Krios#2 and the installation of the Tecnai 12 Biotwin screening microscope. Krios#2 will have a Cs corrector, phase plates and an energy filter, and will be customized for high-throughput cryo-EM including single particle and tomography applications. The addition of these instruments will expand our capacity for high resolution structures and allow greater throughput for optimization of Krios-ready samples.
Preparations for the Krios#3 room have been completed. In November we are scheduled for the delivery of Krios#3 and expect installation to begin early next year. The addition of this microscope is part of SEMC's mission to provide scientists access to high-end instrumentation and support in using electron microscopy in their research programs.
Because of the construction, access to the microscopes continues to be limited. During this time please email EMG@NYSBC.org to schedule microscope use.
Please bear with us during this time. We expect to be able to use our control rooms again soon.
Click here to view an updated renovation schedule.
Click here to learn about scheduling microscope time during the renovation period.
There is new information on our "To Start" page regarding how to obtain the consumable laboratory supplies that users are required provide for their projects. To learn more about it, click here.
Friday, October 28th, 9:00am
Computational Methods for CryoEM Workshop
This workshop will focus on computational approaches for cryoEM. The goal is to bridge between the computational science community in NYC and the cryoEM community.
For registration, visit: https://www.surveymonkey.com/r/QSX6VGH. There will be a limit of 15 attendees.
Tuesday, October 25th, 9:30am RELION Workshop with Sjors Scheres
This one-day workshop will focus on the RELION software package and is led by its developer Sjors Scheres (MRC-LMB). The training will involve a hands-on tutorial for cryo-EM single-particle analysis.
For details about RELION, click here.
Phase Plate Workshop
FEI's volta phase plate has been installed on Krios#1. A SEMC Phase Plate Workshop was held on September 21-22 and led by Rado Danev, MPI Martenstrieg. The workshop included a demonstration of the optimal use of the device and processing of phase plate data.
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