NYSBC - New York Structural Biology Center

Simons Electron Microscopy Center


The Simons Electron Microscopy Center provides expertise and resources for understanding both molecular and cellular structures. Molecular structure determination is enabled by high-end transmission electron microscopes (TEMs), direct detection cameras, and computational support for single particle analysis. Cellular structure determination is enabled by tomographic reconstructions using the TEMs and a focused ion beam (FIB) scanning electron microscope (SEM). SEMC is supported by dues from member institutions as well as the Simons Foundation.


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News

Request For Applications (RFA) for Krios#3 Open Beta

INSTRUMENT: Krios#3 is a 300kV Titan Krios with: 1) Phase Plates and 2) Gatan GIF Quantum Energy Filter.

WHO IS ELIGIBLE: Open beta is designed to streamline our workflows in preparation for normal operations. Mature projects will take higher priority, however all active users from our member institutions are encouraged to apply for EM time. The minimum requirement is for users to have screened similar cryo sample conditions (including preliminary data processing) for the grids that they will be loading into the instrument.

REQUIREMENTS: Interested users should submit a proposal. Users must have: 1) registered as a user, 2) registered their project and, 3) completed a SEMC New User Orientation session (offered once a month). To apply for this orientation email EMG@NYSBC.org.

SUPPORTED WORKFLOWS: Cryo-single particle and cryo-electron tomography workflows on the Gatan K2 Summit in counting mode will be supported.

FEI BioTWIN


BioTWIN The FEI T12 BioTWIN screening microscope is now open for general use. As established for the JEOL1230 screening microscope, member institution users may book time on the instrument within their institutional allocation. The addition of this instrument allows greater throughput for screening and optimization of too temperature and vitrified samples.

Until users are certified to be independent on this instrument they must email EMG@NYSBC.org to request staff assistance concurrent with their reservation.

Laboratory Supplies
All SEMC users must purchase their own consumable EM supplies. Grids, grid boxes, Krios cartridges and small personal tools must be purchased prior to your lab session. We encourage you to buy these directly from the vendors. There is new information on our "To Start" page regarding how to obtain the consumable laboratory supplies that users are required provide for their projects. To learn more about it, click here. If you choose to purchase items using the PayPal method explained in the link, you must bring proof of purchase with you to the lab in order to receive your supplies.

Upcoming/Ongoing Events

August 8th, 2017 - 12:00pm
SEMC FORUM
NYSBC Main Conference Room
Revealing Conformation States of RNA Polymerases
Wei-hau Chang, Yi-Min Wu, and Chun-hsiung Wang
Associate Research Fellows, Institute of Chemistry, Academia Sinica

Wei-hau Chang, Yi-Min Wu, and Chun-hsiung Wang of the Institute of Chemistry, Academia Sinica will be here to a Forum on present Revealing Conformation States of RNA Polymerases. To learn more about it and read their abstract, click here.

October 10th, 2017 - 12:00pm
Appion Part 3: Tomography
SEMC Conference Room

In this workshop users will gain hand-on experience with a semi-automated implementation of Protomo into Appion. To read the full description, click here.

If you are interested in registering for the Appion Part 3 Workshop, follow this link: https://www.surveymonkey.com/r/QXSL789. Registration for the workshop is limited to ten participants.

Recent Events

SEMC Electron Microscopy Winter Course
The Winter Workshop has ended for 2017.

For more information about the course, please click here.

Please email EMG@NYSBC.org to inquire about registering for the next Winter Course.

August 8th, 2017
New User Orientation
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Although New User Orientation is offered on the first Tuesday of every month, August's session was held on the 8th to accommodate another workshop in the lab. The morning session covered covers the general laboratory policies and safety training, and the afternoon session includes a demonstration of TEM use and introduction to Leginon. To inquire about taking the next New User Orientation, please click here.