NYSBC - New York Structural Biology Center

Simons Electron Microscopy Center


The Simons Electron Microscopy Center provides expertise and resources for understanding both molecular and cellular structures. Molecular structure determination is enabled by high-end transmission electron microscopes (TEMs), direct detection cameras, and computational support for single particle analysis. Cellular structure determination is enabled by tomographic reconstructions using the TEMs and a focused ion beam (FIB) scanning electron microscope (SEM). SEMC is supported by dues from member institutions as well as the Simons Foundation.


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Request For Applications (RFA) for Krios#3 Open Beta

INSTRUMENT: Krios#3 is a 300kV Titan Krios with: 1) Phase Plates and 2) Gatan GIF Quantum Energy Filter.

WHO IS ELIGIBLE: Open beta is designed to streamline our workflows in preparation for normal operations. Mature projects will take higher priority, however all active users from our member institutions are encouraged to apply for EM time. The minimum requirement is for users to have screened similar cryo sample conditions (including preliminary data processing) for the grids that they will be loading into the instrument.

REQUIREMENTS: Interested users should submit a proposal. Users must have: 1) registered as a user, 2) registered their project and, 3) completed a SEMC New User Orientation session (offered once a month). To apply for this orientation email EMG@NYSBC.org.

SUPPORTED WORKFLOWS: Cryo-single particle and cryo-electron tomography workflows on the Gatan K2 Summit in counting mode will be supported.

FEI BioTWIN


BioTWIN The FEI T12 BioTWIN screening microscope is now open for general use. As established for the JEOL1230 screening microscope, member institution users may book time on the instrument within their institutional allocation. The addition of this instrument allows greater throughput for screening and optimization of too temperature and vitrified samples.

Until users are certified to be independent on this instrument they must email EMG@NYSBC.org to request staff assistance concurrent with their reservation.

Laboratory Supplies
All SEMC users must purchase their own consumable EM supplies. Grids, grid boxes, Krios cartridges and small personal tools must be purchased prior to your lab session. We encourage you to buy these directly from the vendors. There is new information on our "To Start" page regarding how to obtain the consumable laboratory supplies that users are required provide for their projects. To learn more about it, click here. If you choose to purchase items using the PayPal method explained in the link, you must bring proof of purchase with you to the lab in order to receive your supplies.

Upcoming/Ongoing Events

December 7th, 2017
Appion Part 2: Single Particle Analysis - 3D Reconstruction
This Appion Part 2 Workshop is a follow-on to the Part 1 workshop and will focus on 3D initial models and refinement. If you are interested in attending an Appion Part 2 Workshop, please register at: https://www.surveymonkey.com/r/M96TMBB. Registration is limited to 10 participants.

Recent Events

November 9th, 2017
Appion Part 1: 2D Analysis
The Appion Part 1 Workshop provides hands-on training for the Appion single-particle data processing workflow. Appion is a "pipeline" for processing and analysis of EM images; it is integrated with Leginon data acquisition, but can also be used stand-alone after uploading images or particle stacks. Topics include analyzing the quality of your data collection, correcting the CTF, generating and cleaning up stacks, and generating 2D class averages. If you are interested in participating in this workshop, please register at: https://www.surveymonkey.com/r/FBFR9S2. Registration is limited to 10 participants.


October 10th, 2017
Appion Part 3: Tomography
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In this workshop, users gained hands-on experience with a semi-automated implementation of Protomo into Appion. To read the full description, click here.

If you are interested in registering for the next Appion Part 3 Workshop, please email EMG@NYSBC.org. Registration for the workshop is limited to ten participants.

SEMC Electron Microscopy Winter Course
The Winter Workshop has ended for 2017.

For more information about the course, please click here.

Please email EMG@NYSBC.org to inquire about registering for the next Winter Course.

October 3rd, 2017
New User Orientation
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New User Orientation is offered on the first Tuesday of every month. The morning session covered covers the general laboratory policies and safety training, and the afternoon session includes a demonstration of TEM use and introduction to Leginon. To inquire about taking the next New User Orientation, please click here.