RESOURCES
Electron Microscopes
The microscopes are housed in specially built sound proof rooms, with each microscope placed on a concrete slab that extends to Manhattan bedrock or on a customized vibration table. This construction allows the instruments to be isolated from vibrations or other disturbances commonly found in the city. The walls are sand-filled to keep the rooms quiet. An air conditioning system with duct socks produces near laminar flow, and wall padding minimizes perturbations. The Krios and Helios rooms are additionally fitted with Electro-Magnetic isolation systems.
Transmission Electron Microscopes (TEMs):
TFS Titan Krios #1 “Jim”
FEATURES:
Voltage: 300 kV
Cs: 2.7 mm
Autoloader System
DETECTORS:
Gatan K3 Direct Electron Detector
5,760 x 4,092 pixels
BioQuantum Imaging Filter
Ceta CMOS Detector
4k x 4k pixels
PIXEL SIZES:
64,000x mag: 1.3 Å/pixel
81,000x mag: 1.1 Å/pixel
105,000x mag: 0.8 Å/pixel
TFS Titan Krios #2 “Marilyn”
FEATURES:
Voltage: 300 kV
Cs: 0.001 mm
Autoloader System
Cs Corrector
DETECTORS:
Gatan K3 Direct Electron Detector
5,760 x 4,092 pixels
BioQuantum Imaging Filter
Ceta CMOS Detector
4k x 4k pixels
PIXEL SIZES:
64,000x mag: 1.1 Å/pixel
81,000x mag: 0.8 Å/pixel
105,000x mag: 0.7 Å/pixel
TFS Titan Krios #3 “Audrey”
FEATURES:
Voltage: 300 kV
Cs: 2.7 mm
Volta Phase Plate (VPP)
Autoloader System
DETECTORS:
Falcon III Direct Electron Detector
4k x 4k pixels
Gatan K3 Direct Electron Detector
5,760 x 4,092 pixels
BioQuantum Imaging Filter
Ceta CMOS Detector
4k x 4k pixels
PIXEL SIZES:
64,000x mag: 1.3 Å/pixel
81,000x mag: 1.1 Å/pixel
105,000x mag: 0.8 Å/pixel
TFS Titan Krios #4 “Elizabeth”
FEATURES:
Voltage: 300 kV
Cs: 2.7 mm
Volta Phase Plate (VPP)
Autoloader System
DETECTORS:
Gatan K3 Direct Electron Detector
5,760 x 4,092 pixels
BioQuantum Imaging Filter
Ceta CMOS Detector
4k x 4k pixels
PIXEL SIZES:
64,000x mag: 1.3 Å/pixel
81,000x mag: 1.1 Å/pixel
105,000x mag: 0.8 Å/pixel
TFS Titan Krios #5 “Nathaniel”
FEATURES:
Voltage: 300 kV
Cs: 2.7 mm
Autoloader System
DETECTORS:
Gatan K3 Direct Electron Detector
5,760 x 4,092 pixels
BioQuantum Imaging Filter
Ceta CMOS Detector
4k x 4k pixels
PIXEL SIZES:
81,000x mag: 1.069 Å/pixel
105,000x mag: 0.825 Å/pixel
130,000x mag: 0.67 Å/pixel
TFS Titan Krios #6 “Caitlin”
FEATURES:
Voltage: 300 kV
Cs: 2.7 mm
Autoloader System
DETECTORS:
Gatan K3 Direct Electron Detector
5,760 x 4,092 pixels
BioQuantum Imaging Filter
Ceta CMOS Detector
4k x 4k pixels
PIXEL SIZES:
64,000x mag, native super-res: 0.686 Å/pixel
105,000x mag: 0.889 Å/pixel
130,000x mag: 0.661 Å/pixel
TFS Titan Krios #7 “Matthew”
FEATURES:
Voltage: 300 kV
Cs: 2.7 mm
Volta Phase Plate (VPP)
Autoloader System
DETECTORS:
Falcon IV Direct Electron Detector
4092 x 4092 pixels
Ceta CMOS Detector
4k x 4k pixels
TFS Glacios #1 “Alison”
FEATURES:
Voltage: 200 kV
Cs: 2.7 mm
Autoloader System
DETECTORS:
Falcon III Direct Electron Detector
4k x 4k pixels
Ceta CMOS Detector
4k x 4k pixels
TFS Glacios #2 “Walter”
FEATURES:
Voltage: 200 kV
Cs: 2.7 mm
Autoloader System
DETECTORS:
Falcon III Direct Electron Detector
4k x 4k pixels
Ceta CMOS Detector
4k x 4k pixels
TFS Tundra “Linda”
FEATURES:
Voltage: 100 kV
Cs: 1.6 mm
CLS System
DETECTORS:
Ceta-F CMOS Detector
4k x 4k pixels
Hitachi HT7800 “Marcia”
FEATURES:
Voltage: 20 – 120 kV
RT Three Slot Side Entry Holder
DETECTORS:
TVIPS F416 CMOS Detector
4k x 4k pixels
TFS Aquilos 2 Cryo-FIB DualBeam
FEATURES:
Electron Optics
Beam current: 1.5 pA to 400 nA
Accelerating voltage: 200 V – 30 kV
Resolution (with cryo-stage): 1.6 nm at 30 kV
2.6 nm at 2 kV (RT)
6.0 nm at 2kV (Cryo)
Ion Optics
Voltage: 500 V to 30 kV
Beam current: 1.5 pA – 65 nA in 15 steps
Resolution (with cryo-stage): 7.0 nm at 30 kV
TFS AutoTEM™ Cryo Software
TFS EasyLift™ NanoManipulator cryo-lift-out system
TFS Auto Slice and View™ Software (AS&V)
DETECTORS:
In-lens detection system: segmented lower (T1)
and upper detector (T2)
Everhart-Thornley SE detector (ETD)
IR camera for viewing sample/column
In-chamber TFS Nav-Cam™ Camera
EMCrafts Cube II
FEATURES:
Resolution
5.0nm (SE image)
Magnification
x10 ~ x200,000
Display Modes
Focus: 320 x 240 pixel
Preview: 800 x 600 pixel
Slow: applicable to preview and focus modes
Photo: 3200 x 2400 pixel
DETECTORS:
SE Detector
4CH BSE Detector
Nikon Eclipse Ti U
Epi-Fluorescence Module
Sample Prep:
Chameleon
Sample vitrification instrument
ZEISS LSM 900 with Airyscan 2
Compact Confocal for Multiplex Imaging
Gatan Solarus II
Plasma Cleaner
Leica EM UC6
Ultramicrotome
Computational Resources:
3 PB Parallel File Systems (beegfs/panfs)
Additional equipment:
- Plunge Freezers: TFS Vitrobot, Leica EM GP2
- High Pressure Freezers: Wohlwend HPF Compact 01
- Evaporator: Safematic high compact vacuum coating system
- Mass Photometer: Refeyn One(MP)
- Micropatterning system: Alveole Lab PRIMO
- Inverted Light Microscop: Leica DMi8