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Electron Microscopes

The microscopes are housed in specially built sound proof rooms, with each microscope placed on a concrete slab that extends to Manhattan bedrock or on a customized vibration table. This construction allows the instruments to be isolated from vibrations or other disturbances commonly found in the city. The walls are sand-filled to keep the rooms quiet. An air conditioning system with duct socks produces near laminar flow, and wall padding minimizes perturbations. The Krios and Helios rooms are additionally fitted with Electro-Magnetic isolation systems.

Transmission Electron Microscopes (TEMs):

TFS Titan Krios #1 “Jim”

FEATURES:

Voltage: 300 kV
Cs: 2.7 mm
Autoloader System

DETECTORS:

Gatan K3 Direct Electron Detector
5,760 x 4,092 pixels
BioQuantum Imaging Filter

Ceta CMOS Detector
4k x 4k pixels

PIXEL SIZES:

64,000x mag: 1.3 Å/pixel
81,000x mag: 1.1 Å/pixel
105,000x mag: 0.8 Å/pixel

TFS Titan Krios #2 “Marilyn”

FEATURES:

Voltage: 300 kV
Cs: 0.001 mm
Autoloader System
Cs Corrector

DETECTORS:

Gatan K3 Direct Electron Detector
5,760 x 4,092 pixels
BioQuantum Imaging Filter

Ceta CMOS Detector
4k x 4k pixels

PIXEL SIZES:

64,000x mag: 1.1 Å/pixel
81,000x mag: 0.8 Å/pixel
105,000x mag: 0.7 Å/pixel

TFS Titan Krios #3 “Audrey”

FEATURES:

Voltage: 300 kV
Cs: 2.7 mm
Volta Phase Plate (VPP)
Autoloader System

DETECTORS:

Falcon III Direct Electron Detector
4k x 4k pixels

Gatan K3 Direct Electron Detector
5,760 x 4,092 pixels
BioQuantum Imaging Filter

Ceta CMOS Detector
4k x 4k pixels

PIXEL SIZES:

64,000x mag: 1.3 Å/pixel
81,000x mag: 1.1 Å/pixel
105,000x mag: 0.8 Å/pixel

TFS Titan Krios #4 “Elizabeth”

FEATURES:

Voltage: 300 kV
Cs: 2.7 mm
Volta Phase Plate (VPP)
Autoloader System

DETECTORS:

Gatan K3 Direct Electron Detector
5,760 x 4,092 pixels
BioQuantum Imaging Filter

Ceta CMOS Detector
4k x 4k pixels

PIXEL SIZES:

64,000x mag: 1.3 Å/pixel
81,000x mag: 1.1 Å/pixel
105,000x mag: 0.8 Å/pixel

TFS Titan Krios #5 “Nathaniel”

FEATURES:

Voltage: 300 kV
Cs: 2.7 mm
Autoloader System

DETECTORS:

Gatan K3 Direct Electron Detector
5,760 x 4,092 pixels
BioQuantum Imaging Filter

Ceta CMOS Detector
4k x 4k pixels

PIXEL SIZES:

81,000x mag: 1.069 Å/pixel
105,000x mag: 0.825 Å/pixel
130,000x mag: 0.67 Å/pixel

TFS Titan Krios #6 “Caitlin”

FEATURES:

Voltage: 300 kV
Cs: 2.7 mm
Autoloader System

DETECTORS:

Gatan K3 Direct Electron Detector
5,760 x 4,092 pixels
BioQuantum Imaging Filter

Ceta CMOS Detector
4k x 4k pixels

PIXEL SIZES:

64,000x mag, native super-res: 0.686 Å/pixel
105,000x mag: 0.889 Å/pixel
130,000x mag: 0.661 Å/pixel

TFS Titan Krios #7 “Matthew”

FEATURES:

Voltage: 300 kV
Cs: 2.7 mm
Volta Phase Plate (VPP)
Autoloader System

DETECTORS:

Falcon IV Direct Electron Detector
4092 x 4092 pixels

Ceta CMOS Detector
4k x 4k pixels

TFS Glacios #1 “Alison”

FEATURES:

Voltage: 200 kV
Cs: 2.7 mm
Autoloader System

DETECTORS:

Falcon III Direct Electron Detector
4k x 4k pixels

Ceta CMOS Detector
4k x 4k pixels

TFS Glacios #2 “Walter”

FEATURES:

Voltage: 200 kV
Cs: 2.7 mm
Autoloader System

DETECTORS:

Falcon III Direct Electron Detector
4k x 4k pixels

Ceta CMOS Detector
4k x 4k pixels

TFS Tundra “Linda”

FEATURES:

Voltage: 100 kV
Cs: 1.6 mm
CLS System

DETECTORS:

Ceta-F CMOS Detector
4k x 4k pixels

Hitachi HT7800 “Marcia”

FEATURES:

Voltage: 20 – 120 kV
RT Three Slot Side Entry Holder

DETECTORS:

TVIPS F416 CMOS Detector
4k x 4k pixels

TFS Aquilos 2 Cryo-FIB DualBeam

FEATURES:

Electron Optics
Beam current: 1.5 pA to 400 nA
Accelerating voltage: 200 V – 30 kV
Resolution (with cryo-stage): 1.6 nm at 30 kV
2.6 nm at 2 kV (RT)
6.0 nm at 2kV (Cryo)

Ion Optics
Voltage: 500 V to 30 kV
Beam current: 1.5 pA – 65 nA in 15 steps
Resolution (with cryo-stage): 7.0 nm at 30 kV

TFS AutoTEM™ Cryo Software
TFS EasyLift™ NanoManipulator cryo-lift-out system
TFS Auto Slice and View™ Software (AS&V)

DETECTORS:

In-lens detection system: segmented lower (T1)
and upper detector (T2)
Everhart-Thornley SE detector (ETD)
IR camera for viewing sample/column
In-chamber TFS Nav-Cam™ Camera

EMCrafts Cube II

FEATURES:

Resolution
5.0nm (SE image)

Magnification
x10 ~ x200,000

Display Modes
Focus: 320 x 240 pixel
Preview: 800 x 600 pixel
Slow: applicable to preview and focus modes
Photo: 3200 x 2400 pixel

DETECTORS:

SE Detector
4CH BSE Detector

Nikon Eclipse Ti U

Epi-Fluorescence Module

Sample Prep:

Chameleon

Sample vitrification instrument

ZEISS LSM 900 with Airyscan 2

Compact Confocal for Multiplex Imaging

Gatan Solarus II

Plasma Cleaner

Leica EM UC6

Ultramicrotome

Computational Resources:

3 PB Parallel File Systems (beegfs/panfs)

Additional equipment: